Three novel clade B serine protease inhibitors from disk abalone, Haliotis discus discus

Molecular perspectives and responses to immune challenges and tissue injury

S. D N K Bathige, Navaneethaiyer Umasuthan, G. I. Godahewa, Ilson Whang, Chul Kim, Hae Chul Park, Jehee Lee

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Serine protease inhibitors (SERPINs) control cellular protease activity in order to maintain cellular homeostasis. The immune and inflammatory responses of invertebrate clade B SERPINs have not been widely reported. In the present study, three proteins with high similarity to clade B SERPINs, referred to as AbSERPIN-1, AbSERPIN-2 and AbSERPIN-3, were identified from disk abalone (. Haliotis discus discus). While AbSERPIN-1 (399 aa) was of a typical size for this protein class, AbSERPIN-2 (506 aa) and AbSERPIN-3 (532 aa) were relatively larger. Bioinformatic analysis revealed the characteristic SERPIN domain in each AbSERPIN. In addition, the N-terminal region of both AbSERPIN-2 and AbSERPIN-3 contained a predicted low complexity region (LCR) and a signal peptide, suggesting that these proteins are secretory proteins and are, thus, novel peptides. Tertiary structural models of the AbSERPINs highlighted their structural and functional conservation. Ubiquitous expression of AbSERPIN transcripts was evaluated by quantitative real time PCR (qPCR) analysis in seven tissue types. AbSERPIN-1, AbSERPIN-2, and AbSERPIN-3 transcript levels were highest in mantle, hemocytes, and muscles, respectively. Temporal expression analysis revealed that AbSERPINs were significantly (. P<0.05) elevated in hemocytes during the early/middle stages following the injection of a bacterial pathogen (. Vibrio parahaemolyticus or Listeria monocytogenes) or an immuno-stimulant (lipopolysaccharide). Moreover, mantle tissue injury led to significant changes in the temporal expression of AbSERPIN mRNA. Specifically, transcription of AbSERPIN-1 and AbSERPIN-3 was considerably up-regulated, while expression of AbSERPIN-2 was suppressed. These results suggest a potential role of AbSERPINs in response to pathogen invasion and tissue injury in disk abalone.

Original languageEnglish
Pages (from-to)334-341
Number of pages8
JournalFish and Shellfish Immunology
Volume45
Issue number2
DOIs
Publication statusPublished - 2015 Aug 1

Fingerprint

Haliotis discus
Serine Proteinase Inhibitors
abalone
serine proteinases
proteinase inhibitors
inhibitor
Tissue
protein
Pathogens
hemocytes
peptide
Proteins
proteins
pathogen
mantle
Listeria
bioinformatics
Vibrio parahaemolyticus
pathogens
homeostasis

Keywords

  • Haliotis discus discus
  • Immune response
  • Serine protease inhibitor (SERPIN)
  • Tissue injury

ASJC Scopus subject areas

  • Aquatic Science
  • Environmental Chemistry

Cite this

Three novel clade B serine protease inhibitors from disk abalone, Haliotis discus discus : Molecular perspectives and responses to immune challenges and tissue injury. / Bathige, S. D N K; Umasuthan, Navaneethaiyer; Godahewa, G. I.; Whang, Ilson; Kim, Chul; Park, Hae Chul; Lee, Jehee.

In: Fish and Shellfish Immunology, Vol. 45, No. 2, 01.08.2015, p. 334-341.

Research output: Contribution to journalArticle

Bathige, S. D N K ; Umasuthan, Navaneethaiyer ; Godahewa, G. I. ; Whang, Ilson ; Kim, Chul ; Park, Hae Chul ; Lee, Jehee. / Three novel clade B serine protease inhibitors from disk abalone, Haliotis discus discus : Molecular perspectives and responses to immune challenges and tissue injury. In: Fish and Shellfish Immunology. 2015 ; Vol. 45, No. 2. pp. 334-341.
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