Thrombolytic activity of cheonggukjang kinase in recovery from brain damage in a rat cerebral embolic stroke model

Angella M Anthony Jalin, Chae Kwan Lee, Chae Yeon Lee, A. Reum Kang, Chung Mu Park, Jae Ho Cha, Jeong Hwan Kim, Soo Woong Lee, Young Sun Song, Jong-Tae Lee, Sung Goo Kang

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4 Citations (Scopus)

Abstract

Cheonggukjang is a soybean paste made by fermenting whole cooked soybeans with Bacillus subtilis. Cheonggukjang contains a fibrinolytic enzyme that could provide clinical applications for removing blood clots. In the present study, the term "cheonggukjang kinase" (CGK) was used to refer to this fibrinolytic enzyme. The thrombolytic effects of CGK were analyzed in a rat model of cerebral embolic stroke produced by middle cerebral artery occlusion (MCAO). Results from fibrin and platelet-rich clot lysis assays demonstrated that thrombolytic activity was greatest in CGKs, which were cultured for 40 hours. In addition, T50, the time needed to decompose 50% of the clot, did not change with plasminogen treatment, indicating that CGK was not a plasminogen activator, but was rather presumed to act as a plasmin-like protein. An intravenous infusion of CGK (1 U plasmin-like activity/100 μg CGK/kg) at 1 hour after MCAO resulted in removal of clots in a rat model of cerebral embolic stroke. CGK-treated groups exhibited a significant dose-dependent reduction in infarct volume. CGK treatment also improved functional recovery, as assessed by neurological deficit scores. Decreased infarct volume and improved functional recovery following CGK treatment was greater compared with recombinant tissue plasminogen activator (10 mg/kg). These results suggested that CGK effectively reduced infarct volume and improved functional recovery following ischemic brain injury. CGK exhibits a number of potential clinical applications in the treatment of cerebral embolic stroke.

Original languageEnglish
Pages (from-to)1875-1882
Number of pages8
JournalNeural Regeneration Research
Volume5
Issue number24
Publication statusPublished - 2010 Dec 1
Externally publishedYes

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Keywords

  • Brain damage
  • Cheonggukjang kinase
  • Fibrin clot lysis assay
  • Middle cerebral artery occlusion
  • Platelet rich clot lysis assay
  • Thrombolytic activity

ASJC Scopus subject areas

  • Developmental Neuroscience

Cite this

Jalin, A. M. A., Lee, C. K., Lee, C. Y., Kang, A. R., Park, C. M., Cha, J. H., Kim, J. H., Lee, S. W., Song, Y. S., Lee, J-T., & Kang, S. G. (2010). Thrombolytic activity of cheonggukjang kinase in recovery from brain damage in a rat cerebral embolic stroke model. Neural Regeneration Research, 5(24), 1875-1882.