Time resolved studies of interfacial reactions of ozone with pulmonary phospholipid surfactants using field induced droplet ionization mass spectrometry

Hugh I. Kim, Hyungjun Kim, Young Shik Shin, Luther W. Beegle, William A. Goddard, James R. Heath, Isik Kanik, J. L. Beauchamp

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Field induced droplet ionization mass spectrometry (FIDI-MS) comprises a soft ionization method to sample ions from the surface of microliter droplets. A pulsed electric field stretches neutral droplets until they develop dual Taylor cones, emitting streams of positively and negatively charged submicrometer droplets in opposite directions, with the desired polarity being directed into a mass spectrometer for analysis. This methodology is employed to study the heterogeneous ozonolysis of 1-palmitoyl-2-oleoyl-sn-phosphatidylglycerol (POPG) at the air-liquid interface in negative ion mode using FIDI mass spectrometry. Our results demonstrate unique characteristics of the heterogeneous reactions at the air-liquid interface. We observe the hydroxyhydroperoxide and the secondary ozonide as major products of POPG ozonolysis in the FIDI-MS spectra. These products are metastable and difficult to observe in the bulk phase, using standard electrospray ionization (ESI) for mass spectrometric analysis. We also present studies of the heterogeneous ozonolysis of a mixture of saturated and unsaturated phospholipids at the air-liquid interface. A mixture of the saturated phospholipid 1,2-dipalmitoyl-sn-phosphatidylglycerol (DPPG) and unsaturated POPG is investigated in negative ion mode using FIDI-MS while a mixture of 1,2-dipalmitoyl-sn-phosphatidylcholine (DPPC) and 1-stearoyl-2-oleoyl-sn-phosphatidylcholine (SOPC) surfactant is studied in positive ion mode. In both cases FIDI-MS shows the saturated and unsaturated pulmonary surfactants form a mixed interfacial layer. Only the unsaturated phospholipid reacts with ozone, forming products that are more hydrophilic than the saturated phospholipid. With extensive ozonolysis only the saturated phospholipid remains at the droplet surface. Combining these experimental observations with the results of computational analysis provides an improved understanding of the interfacial structure and chemistry of a surfactant layer system when subject to oxidative stress.

Original languageEnglish
Pages (from-to)9496-9503
Number of pages8
JournalJournal of Physical Chemistry B
Volume114
Issue number29
DOIs
Publication statusPublished - 2010 Jul 29
Externally publishedYes

Fingerprint

Pulmonary Surfactants
Time and motion study
Ozone
Phospholipids
Surface chemistry
Phosphatidylglycerols
Surface-Active Agents
ozone
Ionization
Mass spectrometry
Mass Spectrometry
Surface active agents
mass spectroscopy
surfactants
ionization
liquid air
Ions
Air
negative ions
products

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

Cite this

Time resolved studies of interfacial reactions of ozone with pulmonary phospholipid surfactants using field induced droplet ionization mass spectrometry. / Kim, Hugh I.; Kim, Hyungjun; Shin, Young Shik; Beegle, Luther W.; Goddard, William A.; Heath, James R.; Kanik, Isik; Beauchamp, J. L.

In: Journal of Physical Chemistry B, Vol. 114, No. 29, 29.07.2010, p. 9496-9503.

Research output: Contribution to journalArticle

Kim, Hugh I. ; Kim, Hyungjun ; Shin, Young Shik ; Beegle, Luther W. ; Goddard, William A. ; Heath, James R. ; Kanik, Isik ; Beauchamp, J. L. / Time resolved studies of interfacial reactions of ozone with pulmonary phospholipid surfactants using field induced droplet ionization mass spectrometry. In: Journal of Physical Chemistry B. 2010 ; Vol. 114, No. 29. pp. 9496-9503.
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