Tissue Beads: Tissue-Specific Extracellular Matrix Microbeads to Potentiate Reprogrammed Cell-Based Therapy

Jung Seung Lee, Yoon Ho Roh, Yi Sun Choi, Yoonhee Jin, Eun Je Jeon, Ki Wan Bong, Seung Woo Cho

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Microbeads have been utilized as efficient cell culture carriers and injectable scaffolds for cell transplantation. However, various polymers currently used to generate microbeads have limited applicability due to loss of biological functions and tissue-specific effects. Here, a tissue bead platform is reported that can provide a tissue-specific microenvironment to facilitate cell culture and potentiate cell therapy. Using a flow-focusing microfluidic device, uniform-sized tissue microbeads are fabricated with extracellular matrix (ECM) from various decellularized tissues. The tissue microbeads are tested for tissue-specific encapsulation of induced hepatic (iHep), induced cardiac (iCar), and induced myogenic (iMyo) cells, which are directly reprogrammed from mouse primary fibroblasts. Tissue-specific microbeads significantly enhanced the viability, lineage-specific maturation, and functionality of each type of reprogrammed cell, as compared to functionality when using conventional microbeads from a single ECM component (collagen). Finally, tissue microbeads are confirmed to mediate the successful in vivo engraftment of reprogrammed cells (iHep and iMyo) after transplantation, potentiating cell therapy and promoting functional tissue regeneration in tissue defective animal models. The study suggests that the use of a decellularized tissue matrix combined with a microfluidic technique can be employed to produce tissue-specific ECM microbeads with increased versatility and efficacy for reprogrammed cell-based therapy.

Original languageEnglish
Article number1807803
JournalAdvanced Functional Materials
DOIs
Publication statusPublished - 2019 Jan 1

Fingerprint

beads
therapy
Tissue
matrices
cells
transplantation
Cell culture
Microfluidics
Tissue regeneration
animal models
microfluidic devices
Fibroblasts
Scaffolds (biology)
fibroblasts
Encapsulation
Collagen
collagens
versatility
regeneration
viability

Keywords

  • cell therapy
  • decellularized tissue matrix
  • direct reprogramming
  • tissue-specific microbeads

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics

Cite this

Tissue Beads : Tissue-Specific Extracellular Matrix Microbeads to Potentiate Reprogrammed Cell-Based Therapy. / Lee, Jung Seung; Roh, Yoon Ho; Choi, Yi Sun; Jin, Yoonhee; Jeon, Eun Je; Bong, Ki Wan; Cho, Seung Woo.

In: Advanced Functional Materials, 01.01.2019.

Research output: Contribution to journalArticle

Lee, Jung Seung ; Roh, Yoon Ho ; Choi, Yi Sun ; Jin, Yoonhee ; Jeon, Eun Je ; Bong, Ki Wan ; Cho, Seung Woo. / Tissue Beads : Tissue-Specific Extracellular Matrix Microbeads to Potentiate Reprogrammed Cell-Based Therapy. In: Advanced Functional Materials. 2019.
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