Tissue-specific antioxidant responses in pale chub (Zacco platypus) exposed to copper and benzo[a]pyrene

Woo Keun Kim, June Woo Park, Eun Suk Lim, Sung Kyu Lee, Jungkon Kim, Sunmi Kim, Sang Woo Lee, Kyungho Choi, Jinho Jung

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11 Citations (Scopus)

Abstract

In this study, antioxidant responses including lipid peroxidation (LPO), superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST), were evaluated in the liver, gill and muscle tissues of pale chub (Zacco platypus) exposed to copper (Cu) and benzo[a]pyrene (BaP). Cu exposure induced significant antioxidant responses in Z. platypus, particularly in the liver, whereas BaP exposure had a negligible effect. Following Cu exposure, both SOD and CAT activity increased in a concentration-dependent manner, showing significant correlations with malondialdehyde (MDA) levels as a measure of LPO (r = 0.646 and 0.663, respectively). SOD, CAT and GST mRNA levels were also enhanced following Cu exposure, except at 20 μg L-1, although significant correlations with antioxidant enzyme activities were not found. The results of this study suggest that combined information on SOD and CAT activities together with LPO levels in the liver could be a useful indicator for assessing oxidative stress in freshwater fish.

Original languageEnglish
Pages (from-to)540-545
Number of pages6
JournalBulletin of Environmental Contamination and Toxicology
Volume92
Issue number5
DOIs
Publication statusPublished - 2014 May

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Keywords

  • Catalase
  • Glutathione S-transferase
  • Lipid peroxidation
  • Oxidative stress
  • Superoxide dismutase

ASJC Scopus subject areas

  • Toxicology
  • Pollution
  • Health, Toxicology and Mutagenesis

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