TY - JOUR
T1 - Tissue-specific antioxidant responses in pale chub (Zacco platypus) exposed to copper and benzo[a]pyrene
AU - Kim, Woo Keun
AU - Park, June Woo
AU - Lim, Eun Suk
AU - Lee, Sung Kyu
AU - Kim, Jungkon
AU - Kim, Sunmi
AU - Lee, Sang Woo
AU - Choi, Kyungho
AU - Jung, Jinho
PY - 2014/5
Y1 - 2014/5
N2 - In this study, antioxidant responses including lipid peroxidation (LPO), superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST), were evaluated in the liver, gill and muscle tissues of pale chub (Zacco platypus) exposed to copper (Cu) and benzo[a]pyrene (BaP). Cu exposure induced significant antioxidant responses in Z. platypus, particularly in the liver, whereas BaP exposure had a negligible effect. Following Cu exposure, both SOD and CAT activity increased in a concentration-dependent manner, showing significant correlations with malondialdehyde (MDA) levels as a measure of LPO (r = 0.646 and 0.663, respectively). SOD, CAT and GST mRNA levels were also enhanced following Cu exposure, except at 20 μg L-1, although significant correlations with antioxidant enzyme activities were not found. The results of this study suggest that combined information on SOD and CAT activities together with LPO levels in the liver could be a useful indicator for assessing oxidative stress in freshwater fish.
AB - In this study, antioxidant responses including lipid peroxidation (LPO), superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST), were evaluated in the liver, gill and muscle tissues of pale chub (Zacco platypus) exposed to copper (Cu) and benzo[a]pyrene (BaP). Cu exposure induced significant antioxidant responses in Z. platypus, particularly in the liver, whereas BaP exposure had a negligible effect. Following Cu exposure, both SOD and CAT activity increased in a concentration-dependent manner, showing significant correlations with malondialdehyde (MDA) levels as a measure of LPO (r = 0.646 and 0.663, respectively). SOD, CAT and GST mRNA levels were also enhanced following Cu exposure, except at 20 μg L-1, although significant correlations with antioxidant enzyme activities were not found. The results of this study suggest that combined information on SOD and CAT activities together with LPO levels in the liver could be a useful indicator for assessing oxidative stress in freshwater fish.
KW - Catalase
KW - Glutathione S-transferase
KW - Lipid peroxidation
KW - Oxidative stress
KW - Superoxide dismutase
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U2 - 10.1007/s00128-014-1213-x
DO - 10.1007/s00128-014-1213-x
M3 - Article
C2 - 24477393
AN - SCOPUS:84898830351
VL - 92
SP - 540
EP - 545
JO - Bulletin of Environmental Contamination and Toxicology
JF - Bulletin of Environmental Contamination and Toxicology
SN - 0007-4861
IS - 5
ER -