Tobacco protoplast culture in a polydimethylsiloxane-based microfluidic channel

Jung Moon Ko, Jongil Ju, Sang Hoon Lee, Hyeon Cheol Cha

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Several advances have been made in the use of microfluidic devices for insect and mammalian cell cultures, but no reports of their use for plant cell cultures have been published. We, therefore, conducted a plant cell culture in a microfluidic device using polydimethylsiloxane. Nicotiana tabacum protoplasts were cultured in a variously shaped polydimethylsiloxane channel containing Nitsch medium supplemented with 0.5 g of NLN-13 vitamin mixture, 2.0 mg of α-naphthaleneacetic acid, and 0.5 mg of 6-benzyladenine per liter and 9% mannitol. Protoplasts in the polydimethylsiloxane channel showed cell division and microcolony formation within 4 weeks. The use of a microfluidic channel is a novel technique in the field of plant cell culture. The results of this study will encourage the utilization of polydimethylsiloxane-based microfluidic devices in plant cell engineering and cell analysis.

Original languageEnglish
Pages (from-to)237-240
Number of pages4
JournalProtoplasma
Volume227
Issue number2-4
DOIs
Publication statusPublished - 2006 May

Keywords

  • Microdevice
  • Microfluidic channel
  • Plant cell culture
  • Polydimethylsiloxane

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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