Tobacco Tsip1, a DnaJ-type Zn finger protein, is recruited to and potentiates Tsi1-mediated transcriptional activation

Byung Kook Ham, Jeong Mee Park, Suk Bae Lee, Min Jung Kim, In Ju Lee, Ki Jeong Kim, Chang Seob Kwon, Kyung Hee Paek

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Tobacco stress-induced1 (Tsi1) is an ethylene-responsive-element binding protein/APETALA2-type transcription factor that plays an important role in both biotic and abiotic stress signaling pathways. We show that Tsi1-interacting protein1 (Tsip1), a DnaJ-type Zn finger protein, interacts with Tsi1 in vitro and in yeast (Saccharomyces cerevisiae). The transcript level of Tsip1 in tobacco (Nicotiana tabacum) increased upon treatment with salicylic acid (SA), ethylene, gibberellic acid, NaCl, and virus challenge. Tsip1 appeared to be physically associated with the chloroplast surface but dissociated from it after SA treatment. Tsip1 colocalized and coimmunoprecipitated with Tsi1 in plant cells following SA treatment. Tsip1 expression increased Tsi1-mediated transcription and was able to functionally compensate for loss of the Tsi1 transcriptional activation domain through a direct interaction with Tsi1. Transgenic plants simultaneously coexpressing Tsi1 and Tsip1 displayed stronger pathogen resistance and salt tolerance than did transgenic plants expressing either Tsi1 or Tsip1 alone. Concurrent with this, the expression of a subset of stress-related genes was induced in a cooperative manner in Tsi1/Tsip1 transgenic plants. These results together implied that Tsi1 recruits Tsip1 to the promoters of stress-related genes to potentiate Tsi1-mediated transcriptional activation.

Original languageEnglish
Pages (from-to)2005-2020
Number of pages16
JournalPlant Cell
Volume18
Issue number8
DOIs
Publication statusPublished - 2006 Aug 1

Fingerprint

Tobacco
transcriptional activation
Transcriptional Activation
tobacco
Chemical activation
Proteins
proteins
Salicylic Acid
Genetically Modified Plants
salicylic acid
transgenic plants
Yeast
ethylene
Genes
Salt-Tolerance
plant stress
Pathogens
Transcription
biotic stress
Plant Cells

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Tobacco Tsip1, a DnaJ-type Zn finger protein, is recruited to and potentiates Tsi1-mediated transcriptional activation. / Ham, Byung Kook; Park, Jeong Mee; Lee, Suk Bae; Kim, Min Jung; Lee, In Ju; Kim, Ki Jeong; Kwon, Chang Seob; Paek, Kyung Hee.

In: Plant Cell, Vol. 18, No. 8, 01.08.2006, p. 2005-2020.

Research output: Contribution to journalArticle

Ham, Byung Kook ; Park, Jeong Mee ; Lee, Suk Bae ; Kim, Min Jung ; Lee, In Ju ; Kim, Ki Jeong ; Kwon, Chang Seob ; Paek, Kyung Hee. / Tobacco Tsip1, a DnaJ-type Zn finger protein, is recruited to and potentiates Tsi1-mediated transcriptional activation. In: Plant Cell. 2006 ; Vol. 18, No. 8. pp. 2005-2020.
@article{fa8d3ef682d24b4685046694114a28aa,
title = "Tobacco Tsip1, a DnaJ-type Zn finger protein, is recruited to and potentiates Tsi1-mediated transcriptional activation",
abstract = "Tobacco stress-induced1 (Tsi1) is an ethylene-responsive-element binding protein/APETALA2-type transcription factor that plays an important role in both biotic and abiotic stress signaling pathways. We show that Tsi1-interacting protein1 (Tsip1), a DnaJ-type Zn finger protein, interacts with Tsi1 in vitro and in yeast (Saccharomyces cerevisiae). The transcript level of Tsip1 in tobacco (Nicotiana tabacum) increased upon treatment with salicylic acid (SA), ethylene, gibberellic acid, NaCl, and virus challenge. Tsip1 appeared to be physically associated with the chloroplast surface but dissociated from it after SA treatment. Tsip1 colocalized and coimmunoprecipitated with Tsi1 in plant cells following SA treatment. Tsip1 expression increased Tsi1-mediated transcription and was able to functionally compensate for loss of the Tsi1 transcriptional activation domain through a direct interaction with Tsi1. Transgenic plants simultaneously coexpressing Tsi1 and Tsip1 displayed stronger pathogen resistance and salt tolerance than did transgenic plants expressing either Tsi1 or Tsip1 alone. Concurrent with this, the expression of a subset of stress-related genes was induced in a cooperative manner in Tsi1/Tsip1 transgenic plants. These results together implied that Tsi1 recruits Tsip1 to the promoters of stress-related genes to potentiate Tsi1-mediated transcriptional activation.",
author = "Ham, {Byung Kook} and Park, {Jeong Mee} and Lee, {Suk Bae} and Kim, {Min Jung} and Lee, {In Ju} and Kim, {Ki Jeong} and Kwon, {Chang Seob} and Paek, {Kyung Hee}",
year = "2006",
month = "8",
day = "1",
doi = "10.1105/tpc.106.043158",
language = "English",
volume = "18",
pages = "2005--2020",
journal = "Plant Cell",
issn = "1040-4651",
publisher = "American Society of Plant Biologists",
number = "8",

}

TY - JOUR

T1 - Tobacco Tsip1, a DnaJ-type Zn finger protein, is recruited to and potentiates Tsi1-mediated transcriptional activation

AU - Ham, Byung Kook

AU - Park, Jeong Mee

AU - Lee, Suk Bae

AU - Kim, Min Jung

AU - Lee, In Ju

AU - Kim, Ki Jeong

AU - Kwon, Chang Seob

AU - Paek, Kyung Hee

PY - 2006/8/1

Y1 - 2006/8/1

N2 - Tobacco stress-induced1 (Tsi1) is an ethylene-responsive-element binding protein/APETALA2-type transcription factor that plays an important role in both biotic and abiotic stress signaling pathways. We show that Tsi1-interacting protein1 (Tsip1), a DnaJ-type Zn finger protein, interacts with Tsi1 in vitro and in yeast (Saccharomyces cerevisiae). The transcript level of Tsip1 in tobacco (Nicotiana tabacum) increased upon treatment with salicylic acid (SA), ethylene, gibberellic acid, NaCl, and virus challenge. Tsip1 appeared to be physically associated with the chloroplast surface but dissociated from it after SA treatment. Tsip1 colocalized and coimmunoprecipitated with Tsi1 in plant cells following SA treatment. Tsip1 expression increased Tsi1-mediated transcription and was able to functionally compensate for loss of the Tsi1 transcriptional activation domain through a direct interaction with Tsi1. Transgenic plants simultaneously coexpressing Tsi1 and Tsip1 displayed stronger pathogen resistance and salt tolerance than did transgenic plants expressing either Tsi1 or Tsip1 alone. Concurrent with this, the expression of a subset of stress-related genes was induced in a cooperative manner in Tsi1/Tsip1 transgenic plants. These results together implied that Tsi1 recruits Tsip1 to the promoters of stress-related genes to potentiate Tsi1-mediated transcriptional activation.

AB - Tobacco stress-induced1 (Tsi1) is an ethylene-responsive-element binding protein/APETALA2-type transcription factor that plays an important role in both biotic and abiotic stress signaling pathways. We show that Tsi1-interacting protein1 (Tsip1), a DnaJ-type Zn finger protein, interacts with Tsi1 in vitro and in yeast (Saccharomyces cerevisiae). The transcript level of Tsip1 in tobacco (Nicotiana tabacum) increased upon treatment with salicylic acid (SA), ethylene, gibberellic acid, NaCl, and virus challenge. Tsip1 appeared to be physically associated with the chloroplast surface but dissociated from it after SA treatment. Tsip1 colocalized and coimmunoprecipitated with Tsi1 in plant cells following SA treatment. Tsip1 expression increased Tsi1-mediated transcription and was able to functionally compensate for loss of the Tsi1 transcriptional activation domain through a direct interaction with Tsi1. Transgenic plants simultaneously coexpressing Tsi1 and Tsip1 displayed stronger pathogen resistance and salt tolerance than did transgenic plants expressing either Tsi1 or Tsip1 alone. Concurrent with this, the expression of a subset of stress-related genes was induced in a cooperative manner in Tsi1/Tsip1 transgenic plants. These results together implied that Tsi1 recruits Tsip1 to the promoters of stress-related genes to potentiate Tsi1-mediated transcriptional activation.

UR - http://www.scopus.com/inward/record.url?scp=33747507688&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33747507688&partnerID=8YFLogxK

U2 - 10.1105/tpc.106.043158

DO - 10.1105/tpc.106.043158

M3 - Article

C2 - 16844903

AN - SCOPUS:33747507688

VL - 18

SP - 2005

EP - 2020

JO - Plant Cell

JF - Plant Cell

SN - 1040-4651

IS - 8

ER -