Tocopheryl oligochitosan-based self assembling oligomersomes for siRNA delivery

Sang Myoung Noh, Su Eun Han, Gayong Shim, Kyoung Eun Lee, Chan Wha Kim, Sung Sik Han, Yongseok Choi, Young Keun Kim, Won Ki Kim, Yu Kyoung Oh

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Amphiphilic α-tocopherol oligochitosan conjugates were constructed by conjugating α-tocopherol succinate to water soluble oligochitosans with various molecular weights. In aqueous medium, the tocopherol oligochitosan conjugates self-assembled to single layered oligomersomes. The sizes of α-tocopherol-oligochitosan-based oligomersomes (TCOsomes) could be controlled by chain lengths of oligochitosans. The mean sizes of TCOsomes were 220 and 377 nm as the sizes of oligochitosans were 4000 and 12,500, respectively. For all TCOsomes formed in this study, polydispersity indexes were in the ranges of 0.111-0.256. Cryo-TEM images showed clear thickening in the unilamellar layer of TCOsomes upon complexation with siRNAs. Zeta potentials decreased as the ratios of siRNA/TCOsomes increased. TCOsomes self-assembled from tocopherol-oligochitosan 4K (TCOsome4K) significantly enhanced the cellular uptake of siRNAs (>98%), and reduced the expression of target proteins more effectively than did Lipofectamine 2000. In tumor xenografted mice, the intratumoral administration of siMcl-1 using TCOsomes substantially silenced the expression of Mcl-1 and prevented the growth of tumor. The hematoxylin-eosin staining showed the apoptosis of cells in the tissues of the mice treated with siMcl-1/TCOsome4K complexes, but not with siGL2/TCOsome4K complexes. The self-assembling and size-controllable oligomersomes might be suitable for effective in vivo delivery of siRNAs.

Original languageEnglish
Pages (from-to)849-857
Number of pages9
JournalBiomaterials
Volume32
Issue number3
DOIs
Publication statusPublished - 2011 Jan

Keywords

  • Oligochitosan
  • Oligomersomes
  • Self-assembling
  • SiRNA delivery
  • Tocopherol

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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