Tolerance to acetic acid is improved by mutations of the TATA-binding protein gene

Jieun An, Hyeji Kwon, Eunjung Kim, Young Mi Lee, Hyeok Jin Ko, Hongjae Park, In-Geol Choi, Sooah Kim, Kyoung Heon Kim, Wankee Kim, Wonja Choi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Screening a library of overexpressing mutant alleles of the TATA-binding gene SPT15 yielded two Saccharomyces cerevisiae strains (MRRC 3252 and 3253) with enhanced tolerance to acetic acid. They were also tolerant to propionic acid and hydrogen peroxide. Transcriptome profile analysis identified 58 upregulated genes and 106 downregulated genes in MRRC 3252. Stress- and protein synthesis-related transcription factors were predominantly enriched in the upregulated and downregulated genes respectively. Eight deletion mutants for some of the highly downregulated genes were acetic acid-tolerant. The level of intracellular reactive oxygen species was considerably lessened in MRRC 3252 and 3253 upon exposure to acetic acid. Metabolome profile analysis revealed that intracellular concentrations of 5 and 102 metabolites were increased and decreased, respectively, in MRRC 3252, featuring a large increase of urea and a significant decrease of amino acids. The dur1/2Δmutant, in which the urea degradation gene DUR1/2 is deleted, displayed enhanced tolerance to acetic acid. Enhanced tolerance to acetic acid was also observed on the medium containing a low concentration of amino acids. Taken together, this study identified two SPT15 alleles, nine gene deletions and low concentration of amino acids in the medium that confer enhanced tolerance to acetic acid.

Original languageEnglish
JournalEnvironmental Microbiology
DOIs
Publication statusAccepted/In press - 2014 May 27

Fingerprint

TATA-Box Binding Protein
Acetic Acid
acetic acid
binding proteins
mutation
tolerance
Mutation
protein
gene
Genes
Down-Regulation
genes
amino acid
Amino Acids
amino acids
Urea
urea
Alleles
allele
alleles

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Microbiology

Cite this

An, J., Kwon, H., Kim, E., Lee, Y. M., Ko, H. J., Park, H., ... Choi, W. (Accepted/In press). Tolerance to acetic acid is improved by mutations of the TATA-binding protein gene. Environmental Microbiology. https://doi.org/10.1111/1462-2920.12489

Tolerance to acetic acid is improved by mutations of the TATA-binding protein gene. / An, Jieun; Kwon, Hyeji; Kim, Eunjung; Lee, Young Mi; Ko, Hyeok Jin; Park, Hongjae; Choi, In-Geol; Kim, Sooah; Kim, Kyoung Heon; Kim, Wankee; Choi, Wonja.

In: Environmental Microbiology, 27.05.2014.

Research output: Contribution to journalArticle

An, Jieun ; Kwon, Hyeji ; Kim, Eunjung ; Lee, Young Mi ; Ko, Hyeok Jin ; Park, Hongjae ; Choi, In-Geol ; Kim, Sooah ; Kim, Kyoung Heon ; Kim, Wankee ; Choi, Wonja. / Tolerance to acetic acid is improved by mutations of the TATA-binding protein gene. In: Environmental Microbiology. 2014.
@article{569cb8e2ebaa4a54b7e7c61847a3b910,
title = "Tolerance to acetic acid is improved by mutations of the TATA-binding protein gene",
abstract = "Screening a library of overexpressing mutant alleles of the TATA-binding gene SPT15 yielded two Saccharomyces cerevisiae strains (MRRC 3252 and 3253) with enhanced tolerance to acetic acid. They were also tolerant to propionic acid and hydrogen peroxide. Transcriptome profile analysis identified 58 upregulated genes and 106 downregulated genes in MRRC 3252. Stress- and protein synthesis-related transcription factors were predominantly enriched in the upregulated and downregulated genes respectively. Eight deletion mutants for some of the highly downregulated genes were acetic acid-tolerant. The level of intracellular reactive oxygen species was considerably lessened in MRRC 3252 and 3253 upon exposure to acetic acid. Metabolome profile analysis revealed that intracellular concentrations of 5 and 102 metabolites were increased and decreased, respectively, in MRRC 3252, featuring a large increase of urea and a significant decrease of amino acids. The dur1/2Δmutant, in which the urea degradation gene DUR1/2 is deleted, displayed enhanced tolerance to acetic acid. Enhanced tolerance to acetic acid was also observed on the medium containing a low concentration of amino acids. Taken together, this study identified two SPT15 alleles, nine gene deletions and low concentration of amino acids in the medium that confer enhanced tolerance to acetic acid.",
author = "Jieun An and Hyeji Kwon and Eunjung Kim and Lee, {Young Mi} and Ko, {Hyeok Jin} and Hongjae Park and In-Geol Choi and Sooah Kim and Kim, {Kyoung Heon} and Wankee Kim and Wonja Choi",
year = "2014",
month = "5",
day = "27",
doi = "10.1111/1462-2920.12489",
language = "English",
journal = "Environmental Microbiology",
issn = "1462-2912",
publisher = "Wiley-Blackwell",

}

TY - JOUR

T1 - Tolerance to acetic acid is improved by mutations of the TATA-binding protein gene

AU - An, Jieun

AU - Kwon, Hyeji

AU - Kim, Eunjung

AU - Lee, Young Mi

AU - Ko, Hyeok Jin

AU - Park, Hongjae

AU - Choi, In-Geol

AU - Kim, Sooah

AU - Kim, Kyoung Heon

AU - Kim, Wankee

AU - Choi, Wonja

PY - 2014/5/27

Y1 - 2014/5/27

N2 - Screening a library of overexpressing mutant alleles of the TATA-binding gene SPT15 yielded two Saccharomyces cerevisiae strains (MRRC 3252 and 3253) with enhanced tolerance to acetic acid. They were also tolerant to propionic acid and hydrogen peroxide. Transcriptome profile analysis identified 58 upregulated genes and 106 downregulated genes in MRRC 3252. Stress- and protein synthesis-related transcription factors were predominantly enriched in the upregulated and downregulated genes respectively. Eight deletion mutants for some of the highly downregulated genes were acetic acid-tolerant. The level of intracellular reactive oxygen species was considerably lessened in MRRC 3252 and 3253 upon exposure to acetic acid. Metabolome profile analysis revealed that intracellular concentrations of 5 and 102 metabolites were increased and decreased, respectively, in MRRC 3252, featuring a large increase of urea and a significant decrease of amino acids. The dur1/2Δmutant, in which the urea degradation gene DUR1/2 is deleted, displayed enhanced tolerance to acetic acid. Enhanced tolerance to acetic acid was also observed on the medium containing a low concentration of amino acids. Taken together, this study identified two SPT15 alleles, nine gene deletions and low concentration of amino acids in the medium that confer enhanced tolerance to acetic acid.

AB - Screening a library of overexpressing mutant alleles of the TATA-binding gene SPT15 yielded two Saccharomyces cerevisiae strains (MRRC 3252 and 3253) with enhanced tolerance to acetic acid. They were also tolerant to propionic acid and hydrogen peroxide. Transcriptome profile analysis identified 58 upregulated genes and 106 downregulated genes in MRRC 3252. Stress- and protein synthesis-related transcription factors were predominantly enriched in the upregulated and downregulated genes respectively. Eight deletion mutants for some of the highly downregulated genes were acetic acid-tolerant. The level of intracellular reactive oxygen species was considerably lessened in MRRC 3252 and 3253 upon exposure to acetic acid. Metabolome profile analysis revealed that intracellular concentrations of 5 and 102 metabolites were increased and decreased, respectively, in MRRC 3252, featuring a large increase of urea and a significant decrease of amino acids. The dur1/2Δmutant, in which the urea degradation gene DUR1/2 is deleted, displayed enhanced tolerance to acetic acid. Enhanced tolerance to acetic acid was also observed on the medium containing a low concentration of amino acids. Taken together, this study identified two SPT15 alleles, nine gene deletions and low concentration of amino acids in the medium that confer enhanced tolerance to acetic acid.

UR - http://www.scopus.com/inward/record.url?scp=84900932287&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84900932287&partnerID=8YFLogxK

U2 - 10.1111/1462-2920.12489

DO - 10.1111/1462-2920.12489

M3 - Article

C2 - 24761971

AN - SCOPUS:84925067568

JO - Environmental Microbiology

JF - Environmental Microbiology

SN - 1462-2912

ER -