Tomographic phase microscopy

Wonshik Choi, Christopher Fang-Yen, Kamran Badizadegan, Seungeun Oh, Niyom Lue, Ramachandra R. Dasari, Michael S. Feld

Research output: Contribution to journalArticle

597 Citations (Scopus)

Abstract

We report a technique for quantitative three-dimensional (3D) mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. We demonstrate tomographic imaging of cells and multicellular organisms, and time-dependent changes in cell structure. Our results will permit quantitative characterization of specimen-induced aberrations in high-resolution microscopy and have multiple applications in tissue light scattering.

Original languageEnglish
Pages (from-to)717-719
Number of pages3
JournalNature Methods
Volume4
Issue number9
DOIs
Publication statusPublished - 2007 Sep 1
Externally publishedYes

Fingerprint

Microscopy
Microscopic examination
Tissue
Aberrations
Light scattering
Refractive index
Refractometry
Microscopes
Lighting
Imaging techniques
Lasers
Light

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Cell Biology

Cite this

Choi, W., Fang-Yen, C., Badizadegan, K., Oh, S., Lue, N., Dasari, R. R., & Feld, M. S. (2007). Tomographic phase microscopy. Nature Methods, 4(9), 717-719. https://doi.org/10.1038/nmeth1078

Tomographic phase microscopy. / Choi, Wonshik; Fang-Yen, Christopher; Badizadegan, Kamran; Oh, Seungeun; Lue, Niyom; Dasari, Ramachandra R.; Feld, Michael S.

In: Nature Methods, Vol. 4, No. 9, 01.09.2007, p. 717-719.

Research output: Contribution to journalArticle

Choi, W, Fang-Yen, C, Badizadegan, K, Oh, S, Lue, N, Dasari, RR & Feld, MS 2007, 'Tomographic phase microscopy', Nature Methods, vol. 4, no. 9, pp. 717-719. https://doi.org/10.1038/nmeth1078
Choi W, Fang-Yen C, Badizadegan K, Oh S, Lue N, Dasari RR et al. Tomographic phase microscopy. Nature Methods. 2007 Sep 1;4(9):717-719. https://doi.org/10.1038/nmeth1078
Choi, Wonshik ; Fang-Yen, Christopher ; Badizadegan, Kamran ; Oh, Seungeun ; Lue, Niyom ; Dasari, Ramachandra R. ; Feld, Michael S. / Tomographic phase microscopy. In: Nature Methods. 2007 ; Vol. 4, No. 9. pp. 717-719.
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