Tomographic phase microscopy

Wonshik Choi, Christopher Fang-Yen, Kamran Badizadegan, Seungeun Oh, Niyom Lue, Ramachandra R. Dasari, Michael S. Feld

Research output: Contribution to journalArticle

653 Citations (Scopus)

Abstract

We report a technique for quantitative three-dimensional (3D) mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. We demonstrate tomographic imaging of cells and multicellular organisms, and time-dependent changes in cell structure. Our results will permit quantitative characterization of specimen-induced aberrations in high-resolution microscopy and have multiple applications in tissue light scattering.

Original languageEnglish
Pages (from-to)717-719
Number of pages3
JournalNature Methods
Volume4
Issue number9
DOIs
Publication statusPublished - 2007 Sep

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Choi, W., Fang-Yen, C., Badizadegan, K., Oh, S., Lue, N., Dasari, R. R., & Feld, M. S. (2007). Tomographic phase microscopy. Nature Methods, 4(9), 717-719. https://doi.org/10.1038/nmeth1078