Tomographic phase microscopy and its biological applications

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Conventional interferometric microscopy techniques such as digital holographic microscopy and quantitative phase microscopy are often classified as 3D imaging techniques because a recorded complex field image can be numerically propagated to a different depth. In a strict sense, however, a single complex field image contains only 2D information on a specimen. The measured 2D image is only a subset of the 3D structure. For the 3D mapping of an object, multiple independent 2D images are to be taken, for example at multiple incident angles or wavelengths, and then combined by the so-called optical diffraction tomography (ODT). In this Letter, tomographic phase microscopy (TPM) is reviewed that experimentally realizes the concept of the ODT for the 3D mapping of biological cells in their native state, and some of its interesting biological and biomedical applications are introduced.[Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)1-11
Number of pages11
Journal3D Research
Volume3
Issue number4
DOIs
Publication statusPublished - 2012 Dec 1

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Microscopic examination
Tomography
Diffraction
Imaging techniques
Wavelength

Keywords

  • 3D imaging
  • digital holographic microscopy
  • label-free imaging
  • Optical diffraction tomography

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Software

Cite this

Tomographic phase microscopy and its biological applications. / Choi, Wonshik.

In: 3D Research, Vol. 3, No. 4, 01.12.2012, p. 1-11.

Research output: Contribution to journalArticle

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