Topical application of ALK5 inhibitor A-83-01 reduces burn wound contraction in rats by suppressing myofibroblast population

Xiaoyan Sun, Yang-Hyun Kim, Trong Nhat Phan, Beom Seok Yang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Burn scar contracture that follows the healing of deep dermal burns causes severe deformation and functional impairment. However, its current therapeutic interventions are limited with unsatisfactory outcomes. When we treated deep second-degree burns in rat skin with activin-like kinase 5 (ALK5) inhibitor A-83-01, it reduced wound contraction and enhanced the area of re-epithelialization so that the overall time for wound closing was not altered. In addition, it reduced myofibroblast population in the dermis of burn scar with a diminished deposition of its biomarker proteins such as α-SMA and collagen. Treatment of rat dermal fibroblast with A-83-01 inhibited transforming growth factor-β1 (TGF-β1)- dependent induction of α-SMA and collagen type I. Taken together, these results suggest that topical application of ALK5 inhibitor A-83-01 could be effective in preventing the contraction of burn wound without delaying the wound closure by virtue of its inhibitory activity against the TGF-β-induced increase of myofibroblast population.

Original languageEnglish
Pages (from-to)1805-1812
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume78
Issue number11
DOIs
Publication statusPublished - 2014 Jan 1

Fingerprint

Activins
Myofibroblasts
Rats
Phosphotransferases
Wounds and Injuries
Burns
Skin
Population
Cicatrix
Transforming Growth Factors
Biomarkers
Fibroblasts
Re-Epithelialization
Collagen Type I
Contracture
Collagen
Dermis
A-83-01
Proteins
Therapeutics

Keywords

  • A-83-01
  • ALK5 inhibitor
  • Burn wound contraction
  • Myofibroblast
  • Signaling
  • TGF-β

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Topical application of ALK5 inhibitor A-83-01 reduces burn wound contraction in rats by suppressing myofibroblast population. / Sun, Xiaoyan; Kim, Yang-Hyun; Phan, Trong Nhat; Yang, Beom Seok.

In: Bioscience, Biotechnology and Biochemistry, Vol. 78, No. 11, 01.01.2014, p. 1805-1812.

Research output: Contribution to journalArticle

@article{e5d9333663b944dc986ae33cf3f68a7b,
title = "Topical application of ALK5 inhibitor A-83-01 reduces burn wound contraction in rats by suppressing myofibroblast population",
abstract = "Burn scar contracture that follows the healing of deep dermal burns causes severe deformation and functional impairment. However, its current therapeutic interventions are limited with unsatisfactory outcomes. When we treated deep second-degree burns in rat skin with activin-like kinase 5 (ALK5) inhibitor A-83-01, it reduced wound contraction and enhanced the area of re-epithelialization so that the overall time for wound closing was not altered. In addition, it reduced myofibroblast population in the dermis of burn scar with a diminished deposition of its biomarker proteins such as α-SMA and collagen. Treatment of rat dermal fibroblast with A-83-01 inhibited transforming growth factor-β1 (TGF-β1)- dependent induction of α-SMA and collagen type I. Taken together, these results suggest that topical application of ALK5 inhibitor A-83-01 could be effective in preventing the contraction of burn wound without delaying the wound closure by virtue of its inhibitory activity against the TGF-β-induced increase of myofibroblast population.",
keywords = "A-83-01, ALK5 inhibitor, Burn wound contraction, Myofibroblast, Signaling, TGF-β",
author = "Xiaoyan Sun and Yang-Hyun Kim and Phan, {Trong Nhat} and Yang, {Beom Seok}",
year = "2014",
month = "1",
day = "1",
doi = "10.1080/09168451.2014.932666",
language = "English",
volume = "78",
pages = "1805--1812",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "0916-8451",
publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
number = "11",

}

TY - JOUR

T1 - Topical application of ALK5 inhibitor A-83-01 reduces burn wound contraction in rats by suppressing myofibroblast population

AU - Sun, Xiaoyan

AU - Kim, Yang-Hyun

AU - Phan, Trong Nhat

AU - Yang, Beom Seok

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Burn scar contracture that follows the healing of deep dermal burns causes severe deformation and functional impairment. However, its current therapeutic interventions are limited with unsatisfactory outcomes. When we treated deep second-degree burns in rat skin with activin-like kinase 5 (ALK5) inhibitor A-83-01, it reduced wound contraction and enhanced the area of re-epithelialization so that the overall time for wound closing was not altered. In addition, it reduced myofibroblast population in the dermis of burn scar with a diminished deposition of its biomarker proteins such as α-SMA and collagen. Treatment of rat dermal fibroblast with A-83-01 inhibited transforming growth factor-β1 (TGF-β1)- dependent induction of α-SMA and collagen type I. Taken together, these results suggest that topical application of ALK5 inhibitor A-83-01 could be effective in preventing the contraction of burn wound without delaying the wound closure by virtue of its inhibitory activity against the TGF-β-induced increase of myofibroblast population.

AB - Burn scar contracture that follows the healing of deep dermal burns causes severe deformation and functional impairment. However, its current therapeutic interventions are limited with unsatisfactory outcomes. When we treated deep second-degree burns in rat skin with activin-like kinase 5 (ALK5) inhibitor A-83-01, it reduced wound contraction and enhanced the area of re-epithelialization so that the overall time for wound closing was not altered. In addition, it reduced myofibroblast population in the dermis of burn scar with a diminished deposition of its biomarker proteins such as α-SMA and collagen. Treatment of rat dermal fibroblast with A-83-01 inhibited transforming growth factor-β1 (TGF-β1)- dependent induction of α-SMA and collagen type I. Taken together, these results suggest that topical application of ALK5 inhibitor A-83-01 could be effective in preventing the contraction of burn wound without delaying the wound closure by virtue of its inhibitory activity against the TGF-β-induced increase of myofibroblast population.

KW - A-83-01

KW - ALK5 inhibitor

KW - Burn wound contraction

KW - Myofibroblast

KW - Signaling

KW - TGF-β

UR - http://www.scopus.com/inward/record.url?scp=84938524268&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84938524268&partnerID=8YFLogxK

U2 - 10.1080/09168451.2014.932666

DO - 10.1080/09168451.2014.932666

M3 - Article

VL - 78

SP - 1805

EP - 1812

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 11

ER -