TOPLESS mediates brassinosteroid-induced transcriptional repression through interaction with BZR1

Eunkyoo Oh, Jia Ying Zhu, Hojin Ryu, Ildoo Hwang, Zhi Yong Wang

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Brassinosteroid (BR) regulates plant development by activating the transcription factor brassinazole resistant 1 (BZR1), which activates and represses different target genes to switch cellular programmes. The mechanisms that determine BZR1â ™ s transcriptional activities remain largely unknown. Here we show that BZR1 represses target genes by recruiting the Groucho/TUP1-like transcriptional corepressor TOPLESS (TPL). Specific deletion or mutation of an evolutionarily conserved ERF-associated amphiphilic repression (EAR) motif at the carboxy terminus abolishes BZR1â ™ s abilities to regulate gene expression and cell elongation, but these defects are rescued by TPL fusion to the EAR motif-mutated BZR1. The EAR motif in BZR1 mediates recruitment of TPL to BZR1-repressed promoters. A triple tpl mutant (tpl;tpr1;tpr4) shows reduced BR sensitivity and suppresses the gain-of-function bzr1-1D mutant phenotype. BR repression of gene expression also requires histone deacetylases that interact with TPL. Our study demonstrates key roles of the EAR motif and TPL in BR regulation of gene expression and plant growth.

Original languageEnglish
Article number4140
JournalNature communications
Volume5
DOIs
Publication statusPublished - 2014 Jun 18
Externally publishedYes

Fingerprint

Brassinosteroids
gene expression
genes
Gene expression
deletion
phenotype
interactions
mutations
elongation
Plant Gene Expression Regulation
fusion
Genes
Switch Genes
Activating Transcription Factors
sensitivity
Gene Expression
defects
Co-Repressor Proteins
Histone Deacetylases
Plant Development

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

TOPLESS mediates brassinosteroid-induced transcriptional repression through interaction with BZR1. / Oh, Eunkyoo; Zhu, Jia Ying; Ryu, Hojin; Hwang, Ildoo; Wang, Zhi Yong.

In: Nature communications, Vol. 5, 4140, 18.06.2014.

Research output: Contribution to journalArticle

Oh, Eunkyoo ; Zhu, Jia Ying ; Ryu, Hojin ; Hwang, Ildoo ; Wang, Zhi Yong. / TOPLESS mediates brassinosteroid-induced transcriptional repression through interaction with BZR1. In: Nature communications. 2014 ; Vol. 5.
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