Toward a chemical marker for inflammatory disease: A fluorescent probe for membrane-localized thioredoxin

Min Hee Lee, Hyun Mi Jeon, Ji Hye Han, Nayoung Park, Chulhun Kang, Jonathan L. Sessler, Jong Seung Kim

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Thioredoxin (Trx) is a redox-active protein that plays a key role in mitigating the effects of oxidative stress. The secretion of Trx on the plasma membrane has been suggested as a distinctive feature of inflammation. However, selective monitoring of membrane-associated Trx activity has proved challenging because of the ubiquity of Trx action in cells. Here, we report a Trx-specific probe that allows visualization of Trx activity associated with the membranes via fluorescence microscopy. The ability of this probe to act as a possible screening tool for agents that modulate Trx secretion was demonstrated in HeLa cells under oxidative stress conditions and in a cellular hepatosteatosis model. Control experiments serve to confirm that the response seen for the present probe is due to Trx and that it is selective over various potentially competing metabolites, including thiol-containing small molecules and test proteins.

Original languageEnglish
Pages (from-to)8430-8437
Number of pages8
JournalJournal of the American Chemical Society
Volume136
Issue number23
DOIs
Publication statusPublished - 2014 Jun 11

Fingerprint

Thioredoxins
Fluorescent Dyes
Oxidative stress
Membranes
Proteins
Fluorescence microscopy
Cell membranes
Metabolites
Screening
Visualization
Oxidative Stress
Molecules
Monitoring
Experiments
HeLa Cells
Fluorescence Microscopy
Sulfhydryl Compounds
Oxidation-Reduction
Cell Membrane
Inflammation

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Toward a chemical marker for inflammatory disease : A fluorescent probe for membrane-localized thioredoxin. / Lee, Min Hee; Jeon, Hyun Mi; Han, Ji Hye; Park, Nayoung; Kang, Chulhun; Sessler, Jonathan L.; Kim, Jong Seung.

In: Journal of the American Chemical Society, Vol. 136, No. 23, 11.06.2014, p. 8430-8437.

Research output: Contribution to journalArticle

Lee, Min Hee ; Jeon, Hyun Mi ; Han, Ji Hye ; Park, Nayoung ; Kang, Chulhun ; Sessler, Jonathan L. ; Kim, Jong Seung. / Toward a chemical marker for inflammatory disease : A fluorescent probe for membrane-localized thioredoxin. In: Journal of the American Chemical Society. 2014 ; Vol. 136, No. 23. pp. 8430-8437.
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