Toward the production of flavone-7-O-β-d-glucopyranosides using Arabidopsis glycosyltransferase in Escherichia coli

Nguyen Huy Thuan, Je Won Park, Jae Kyung Sohng

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Flavonoid glycosides are highly attractive targets due to their dominant roles in clinical, cosmetic production and in the food industry. In this research, an Escherichia coli strain bearing the reconstructed uridine-diphosphate glucose (UDP-glucose) pathway cassette and a putative glycosyltransferase from Arabidopsis thaliana, was developed as a host for the production of apigenin-7-O-β-d-glucoside (APG) and baicalein-7-O-β-d- glucoside (BCG) from exogenously supplied flavone aglycones (apigenin and baicalein, respectively). In order to improve the yield, genetic engineering of E. coli strains for optimization of intracellular UDP-glucose generation, as well as media optimization were carried out. The production was scaled up using a fed batch fermentation, and the maximal yield of products reached 90.88 μM (39.28 mg L-1) and 76.82 μM (33.19 mg L-1) of APG and BCG, respectively. And, the maximum bioconversion rate corresponded to 90.88% and 76.82% of apigenin and baicalein, respectively.

Original languageEnglish
Pages (from-to)1744-1748
Number of pages5
JournalProcess Biochemistry
Volume48
Issue number11
DOIs
Publication statusPublished - 2013 Nov 1
Externally publishedYes

Fingerprint

flavone
Apigenin
Glycosyltransferases
Arabidopsis
Escherichia coli
Uridine Diphosphate Glucose
Glucosides
Mycobacterium bovis
Glucose
Bearings (structural)
Glycosides
Genetic engineering
Flavonoids
Bioconversion
Genetic Engineering
Cosmetics
Food Industry
Fermentation
Research
baicalein

Keywords

  • Escherichia coli
  • Flavones
  • Glucosylation
  • Heterologous production
  • Whole cell biotransformation

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

Cite this

Toward the production of flavone-7-O-β-d-glucopyranosides using Arabidopsis glycosyltransferase in Escherichia coli. / Thuan, Nguyen Huy; Park, Je Won; Sohng, Jae Kyung.

In: Process Biochemistry, Vol. 48, No. 11, 01.11.2013, p. 1744-1748.

Research output: Contribution to journalArticle

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