TY - JOUR
T1 - Toxic effects of titanium dioxide nanoparticles on microbial activity and metabolic flux
AU - Lee, Jinwon
AU - Park, Soohyun
AU - Lee, Soojin
AU - Kim, Borim
AU - Lee, Sunhee
AU - Lei, Juhee
AU - Sim, Sangjun
AU - Gu, Manbock
AU - Yi, Jongheop
N1 - Funding Information:
This work was supported by the Ministry of Environment as “The Eco-technopia 21 project” and by the Second Stage of Brain Korea 21 Project (Ministry of Education & Human Resources Development). This research was supported by the R&D Program of MKE/KEIT (No. 10035578, Development of 2,3-butanediol and derivative production technology for C-Zero bio-platform industry). This work was supported by the Graduate School of Specialization for Biotechnology Program of the Ministry of Knowledge Economy (MKE).
PY - 2012/4
Y1 - 2012/4
N2 - The purpose of this research is to estimate and quantify the toxicity of titanium dioxide (TiO2) nanoparticles in microorganisms. Nano-sized particles of TiO2 were more toxic compared to micro-sized particles. Three microorganismal species, Escherichia coli, Bacillus subtilis, and Saccharomyces cerevisiae, were used to test TiO2 antimicrobial effects. E. coli showed the lowest survival rate (36%), while S. cerevisiae showed the highest survival rate (71%). The antimicrobial effect of TiO 2 was also dependent on ultraviolet ray wavelength. The survival ratio of E. coli was 40% at a 254 nm wavelength and 80% at 365 nm. To observe the effect of TiO2 on the intracellular metabolism, a metabolic flux analysis and the measurement of in vivo glucose-6-phosphate were performed. G6P concentration in cells exposed to TiO2 increased, and glycolysis flux was also higher than the controls.
AB - The purpose of this research is to estimate and quantify the toxicity of titanium dioxide (TiO2) nanoparticles in microorganisms. Nano-sized particles of TiO2 were more toxic compared to micro-sized particles. Three microorganismal species, Escherichia coli, Bacillus subtilis, and Saccharomyces cerevisiae, were used to test TiO2 antimicrobial effects. E. coli showed the lowest survival rate (36%), while S. cerevisiae showed the highest survival rate (71%). The antimicrobial effect of TiO 2 was also dependent on ultraviolet ray wavelength. The survival ratio of E. coli was 40% at a 254 nm wavelength and 80% at 365 nm. To observe the effect of TiO2 on the intracellular metabolism, a metabolic flux analysis and the measurement of in vivo glucose-6-phosphate were performed. G6P concentration in cells exposed to TiO2 increased, and glycolysis flux was also higher than the controls.
KW - Antimicrobial activity
KW - Glucose-6-phosphate
KW - Metabolic flux analysis
KW - TiO2 nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=84861048790&partnerID=8YFLogxK
U2 - 10.1007/s12257-010-0251-4
DO - 10.1007/s12257-010-0251-4
M3 - Article
AN - SCOPUS:84861048790
SN - 1226-8372
VL - 17
SP - 276
EP - 282
JO - Biotechnology and Bioprocess Engineering
JF - Biotechnology and Bioprocess Engineering
IS - 2
ER -