Toxicity biomonitoring of degradation byproducts using freeze-dried recombinant bioluminescent bacteria

Sue Hyung Choi, Man Bock Gu

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A toxicity biomonitoring system using freeze-dried recombinant bioluminescent bacteria was implemented to diagnose the biotreatment of 2,4,5-trichlorophenol and its degradation byproducts of using a cell-free culture broth of Phanerochaete chrysosporium. The cellular toxicity was measured by the bioluminescence of constitutive bioluminescent bacteria, such as Photobacterium phosphoreum and GC2, while information on the toxicity caused by unknown byproducts was obtained using the specific bioluminescent responses of stress-inducible bioluminescent bacteria, which included DPD2540 (membrane-damage sensitive), TV1061 (protein-damage sensitive), DPD2794 (DNA-damage sensitive), and DPD2511 (oxidative-damage sensitive) strains. An overall decrease in the cellular toxicity was observed as the treatment progressed, i.e. as 2,4,5-trichlorophenol disappeared. On the other hand, bioluminescent responses from DPD2511, DPD2540, and TV1061 increased as degradation progressed, most probably due to the formation of byproducts causing oxidative-, membrane-, and protein-damage. In conclusion, this toxicity biomonitoring method may be applied to evaluate the toxicities of degradation byproducts of the other environmental processes to provide more information about the mode of the byproducts' toxicity.

Original languageEnglish
Pages (from-to)229-238
Number of pages10
JournalAnalytica Chimica Acta
Volume481
Issue number2
DOIs
Publication statusPublished - 2003 Apr 3
Externally publishedYes

Fingerprint

Environmental Monitoring
biomonitoring
Byproducts
Toxicity
Bacteria
toxicity
Degradation
degradation
bacterium
Photobacterium
Phanerochaete
damage
DNA Damage
Membrane Proteins
Cell Culture Techniques
Membranes
Bioluminescence
membrane
bioluminescence
protein

Keywords

  • Byproducts' toxicity
  • Diagnostic of biotreatment results
  • Freeze-dried bioluminescent bacteria
  • Toxicity classification
  • Toxicity monitoring

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

Toxicity biomonitoring of degradation byproducts using freeze-dried recombinant bioluminescent bacteria. / Choi, Sue Hyung; Gu, Man Bock.

In: Analytica Chimica Acta, Vol. 481, No. 2, 03.04.2003, p. 229-238.

Research output: Contribution to journalArticle

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