Toxicity evaluation and biodegradation of phenanthrene by laccase from Trametes polyzona PBURU 12

Retno Wulandari, Pongtharin Lotrakul, Hunsa Punnapayak, Rudianto Amirta, Seung Wook Kim, Sehanat Prasongsuk

Research output: Contribution to journalArticlepeer-review

Abstract

The newly isolated Trametes polyzona PBURU 12 demonstrated a high tolerance and potential for the degradation of phenanthrene. The fungal isolate was able to tolerate 100 ppm of phenanthrene with 45% relative growth. The crude laccase produced by Trametes polyzona PBURU 12 was able to degrade phenanthrene by up to 98% within 24 h. The degradation metabolites showed the absence of toxic compounds. Microbial viability tests using E. coli and B. subtilis revealed that the treated phenanthrene was less toxic than untreated phenanthrene. Phytotoxicity and genotoxicity tests, using Vigna radiata and Allium cepa, indicated that the treated phenanthrene was less toxic to the plants. No mutagenic activity was found in the Ames test. The crude laccase from Trametes polyzona PBURU 12 was demonstrated as a potential tool for the biodegradation of PAHs (phenanthrene), with low toxic effects after the degradation.

Original languageEnglish
Article number32
Journal3 Biotech
Volume11
Issue number1
DOIs
Publication statusPublished - 2021 Jan

Keywords

  • Biodegradation
  • Lignolytic enzyme
  • Phenanthrene
  • White-rot

ASJC Scopus subject areas

  • Biotechnology
  • Environmental Science (miscellaneous)
  • Agricultural and Biological Sciences (miscellaneous)

Fingerprint Dive into the research topics of 'Toxicity evaluation and biodegradation of phenanthrene by laccase from Trametes polyzona PBURU 12'. Together they form a unique fingerprint.

Cite this