TY - JOUR
T1 - Toxicity monitoring and classification of endocrine disrupting chemicals (EDCs) using recombinant bioluminescent bacteria
AU - Gu, Man Bock
AU - Min, Jiho
AU - Kim, Eun Jin
N1 - Funding Information:
The authors wish to thank Dr. Robert A. LaRossa in DuPont Co. (USA) for the strains used in this study. This work was supported by the Minister of the Environment (G7) through the G7 project, and in part by KOSEF through the Advanced Environmental Monitoring Research Center (ADEMRC) at the Kwangju Institute of Science and Technology (K-JIST). The authors are grateful for their support.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2002
Y1 - 2002
N2 - A recombinant bioluminescent Escherichia coli, DPD2794, containing the recA promoter region fused to luxCDABE originating from Vibrio fischeri was used for detecting genotoxicity caused by endocrine disrupting chemicals (EDCs) to cells. As well, several other recombinant bioluminescent bacteria, including TV1061, which is sensitive to protein damage (grpE::luxCDABE), DPD2511, sensitive to oxidative damage (katG::luxCDABE), and DPD2540, sensitive to membrane damage (fabA::luxCDABE), were used for evaluating other possible modes of toxicity. It was found that the recombinant bacteria could monitor the toxic effect, not estrogenic effect, due to the presence of various EDCs through the measurement of bioluminescence (BL) and cell growth rate, which depend upon the type of toxicity occurring and which of the four strains was used. In addition, it was found that the damage caused by EDCs can be classified into several groups upon their mechanisms of toxic action.
AB - A recombinant bioluminescent Escherichia coli, DPD2794, containing the recA promoter region fused to luxCDABE originating from Vibrio fischeri was used for detecting genotoxicity caused by endocrine disrupting chemicals (EDCs) to cells. As well, several other recombinant bioluminescent bacteria, including TV1061, which is sensitive to protein damage (grpE::luxCDABE), DPD2511, sensitive to oxidative damage (katG::luxCDABE), and DPD2540, sensitive to membrane damage (fabA::luxCDABE), were used for evaluating other possible modes of toxicity. It was found that the recombinant bacteria could monitor the toxic effect, not estrogenic effect, due to the presence of various EDCs through the measurement of bioluminescence (BL) and cell growth rate, which depend upon the type of toxicity occurring and which of the four strains was used. In addition, it was found that the damage caused by EDCs can be classified into several groups upon their mechanisms of toxic action.
KW - Cellular toxicity
KW - Endocrine-disrupting chemicals
KW - Recombinant bioluminescent bacteria
KW - Toxic effect
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U2 - 10.1016/S0045-6535(01)00081-9
DO - 10.1016/S0045-6535(01)00081-9
M3 - Article
C2 - 11827287
AN - SCOPUS:0035993489
VL - 46
SP - 289
EP - 294
JO - Chemosphere
JF - Chemosphere
SN - 0045-6535
IS - 2
ER -