Abstract
A recombinant bioluminescent Escherichia coli, DPD2794, containing the recA promoter region fused to luxCDABE originating from Vibrio fischeri was used for detecting genotoxicity caused by endocrine disrupting chemicals (EDCs) to cells. As well, several other recombinant bioluminescent bacteria, including TV1061, which is sensitive to protein damage (grpE::luxCDABE), DPD2511, sensitive to oxidative damage (katG::luxCDABE), and DPD2540, sensitive to membrane damage (fabA::luxCDABE), were used for evaluating other possible modes of toxicity. It was found that the recombinant bacteria could monitor the toxic effect, not estrogenic effect, due to the presence of various EDCs through the measurement of bioluminescence (BL) and cell growth rate, which depend upon the type of toxicity occurring and which of the four strains was used. In addition, it was found that the damage caused by EDCs can be classified into several groups upon their mechanisms of toxic action.
| Original language | English |
|---|---|
| Pages (from-to) | 289-294 |
| Number of pages | 6 |
| Journal | Chemosphere |
| Volume | 46 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2002 Jan 29 |
| Externally published | Yes |
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Keywords
- Cellular toxicity
- Endocrine-disrupting chemicals
- Recombinant bioluminescent bacteria
- Toxic effect
ASJC Scopus subject areas
- Environmental Science(all)
- Environmental Chemistry
Cite this
Toxicity monitoring and classification of endocrine disrupting chemicals (EDCs) using recombinant bioluminescent bacteria. / Gu, Man Bock; Min, Jiho; Kim, Eun Jin.
In: Chemosphere, Vol. 46, No. 2, 29.01.2002, p. 289-294.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Toxicity monitoring and classification of endocrine disrupting chemicals (EDCs) using recombinant bioluminescent bacteria
AU - Gu, Man Bock
AU - Min, Jiho
AU - Kim, Eun Jin
PY - 2002/1/29
Y1 - 2002/1/29
N2 - A recombinant bioluminescent Escherichia coli, DPD2794, containing the recA promoter region fused to luxCDABE originating from Vibrio fischeri was used for detecting genotoxicity caused by endocrine disrupting chemicals (EDCs) to cells. As well, several other recombinant bioluminescent bacteria, including TV1061, which is sensitive to protein damage (grpE::luxCDABE), DPD2511, sensitive to oxidative damage (katG::luxCDABE), and DPD2540, sensitive to membrane damage (fabA::luxCDABE), were used for evaluating other possible modes of toxicity. It was found that the recombinant bacteria could monitor the toxic effect, not estrogenic effect, due to the presence of various EDCs through the measurement of bioluminescence (BL) and cell growth rate, which depend upon the type of toxicity occurring and which of the four strains was used. In addition, it was found that the damage caused by EDCs can be classified into several groups upon their mechanisms of toxic action.
AB - A recombinant bioluminescent Escherichia coli, DPD2794, containing the recA promoter region fused to luxCDABE originating from Vibrio fischeri was used for detecting genotoxicity caused by endocrine disrupting chemicals (EDCs) to cells. As well, several other recombinant bioluminescent bacteria, including TV1061, which is sensitive to protein damage (grpE::luxCDABE), DPD2511, sensitive to oxidative damage (katG::luxCDABE), and DPD2540, sensitive to membrane damage (fabA::luxCDABE), were used for evaluating other possible modes of toxicity. It was found that the recombinant bacteria could monitor the toxic effect, not estrogenic effect, due to the presence of various EDCs through the measurement of bioluminescence (BL) and cell growth rate, which depend upon the type of toxicity occurring and which of the four strains was used. In addition, it was found that the damage caused by EDCs can be classified into several groups upon their mechanisms of toxic action.
KW - Cellular toxicity
KW - Endocrine-disrupting chemicals
KW - Recombinant bioluminescent bacteria
KW - Toxic effect
UR - http://www.scopus.com/inward/record.url?scp=0035993489&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035993489&partnerID=8YFLogxK
U2 - 10.1016/S0045-6535(01)00081-9
DO - 10.1016/S0045-6535(01)00081-9
M3 - Article
VL - 46
SP - 289
EP - 294
JO - Chemosphere
JF - Chemosphere
SN - 0045-6535
IS - 2
ER -