Toxicity monitoring and classification of endocrine disrupting chemicals (EDCs) using recombinant bioluminescent bacteria

Man Bock Gu, Jiho Min, Eun Jin Kim

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

A recombinant bioluminescent Escherichia coli, DPD2794, containing the recA promoter region fused to luxCDABE originating from Vibrio fischeri was used for detecting genotoxicity caused by endocrine disrupting chemicals (EDCs) to cells. As well, several other recombinant bioluminescent bacteria, including TV1061, which is sensitive to protein damage (grpE::luxCDABE), DPD2511, sensitive to oxidative damage (katG::luxCDABE), and DPD2540, sensitive to membrane damage (fabA::luxCDABE), were used for evaluating other possible modes of toxicity. It was found that the recombinant bacteria could monitor the toxic effect, not estrogenic effect, due to the presence of various EDCs through the measurement of bioluminescence (BL) and cell growth rate, which depend upon the type of toxicity occurring and which of the four strains was used. In addition, it was found that the damage caused by EDCs can be classified into several groups upon their mechanisms of toxic action.

Original languageEnglish
Pages (from-to)289-294
Number of pages6
JournalChemosphere
Volume46
Issue number2
DOIs
Publication statusPublished - 2002 Jan 29
Externally publishedYes

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Keywords

  • Cellular toxicity
  • Endocrine-disrupting chemicals
  • Recombinant bioluminescent bacteria
  • Toxic effect

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry

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