Toxicity monitoring of endocrine disrupting chemicals (EDCs) using freeze-dried recombinant bioluminescent bacteria

Sung Woo Kim, Sue Hyung Choi, Jiho Min, Man Bock Gu

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


Five different freeze-dried recombinant bioluminescent bacteria were used for the detection of cellular stresses caused by endocrine disrupting chemicals. These strains were DPD2794 (recA::luxCDABE), which is sensitive to DNA damage, DPD2540 (fabA:: luxCDABE), sensitive to cellular membrane damage, DPD2511 (katG::luxCDABE), sensitive to oxidative damage, and TV1061 (grpE::luxCDABE), sensitive to protein damage. GC2, which emits bioluminescence constitutively, was also used in this study. The toxicity of several chemicals was determined on the first four freeze-dried bacteria, while nonspecific cellular stresses were measured using GC2. Damage caused by known endocrine disrupting chemicals, such as nonyl phenol, bisphenol A, and styrene, was detected and classified according to toxicity mode, while others, such as phathalate and DDT, were not detected with the bacteria. These results suggest that endocrine disrupting chemicals are toxic in bacteria, and do not act via an estrogenic effect, and that toxicity monitoring and classification of some endocrine dis-rupting chemicals may be possible in the field using these freeze-dried recombinant bioluminescent bacteria.

Original languageEnglish
Pages (from-to)395-399
Number of pages5
JournalBiotechnology and Bioprocess Engineering
Issue number6
Publication statusPublished - 2000
Externally publishedYes


  • Cellular toxicity
  • Endocrine disrupting chemicals (EDCs)
  • Freeze-dried recombinant bioluminescent bacteria
  • Toxic effect

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering


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