TY - JOUR
T1 - Toxicity monitoring of endocrine disrupting chemicals (EDCs) using freeze-dried recombinant bioluminescent bacteria
AU - Kim, Sung Woo
AU - Choi, Sue Hyung
AU - Min, Jiho
AU - Gu, Man Bock
N1 - Funding Information:
Acknowledgements This work was supported by the Minister of the Environment of Korea through the G7 project, and in part by KOSEF through the Advanced Environmental Monitoring Research Center (ADEMRC) at the Kwangju Institute of Science and Technology (K-JIST). Authors are grateful for their support.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2000
Y1 - 2000
N2 - Five different freeze-dried recombinant bioluminescent bacteria were used for the detection of cellular stresses caused by endocrine disrupting chemicals. These strains were DPD2794 (recA::luxCDABE), which is sensitive to DNA damage, DPD2540 (fabA:: luxCDABE), sensitive to cellular membrane damage, DPD2511 (katG::luxCDABE), sensitive to oxidative damage, and TV1061 (grpE::luxCDABE), sensitive to protein damage. GC2, which emits bioluminescence constitutively, was also used in this study. The toxicity of several chemicals was determined on the first four freeze-dried bacteria, while nonspecific cellular stresses were measured using GC2. Damage caused by known endocrine disrupting chemicals, such as nonyl phenol, bisphenol A, and styrene, was detected and classified according to toxicity mode, while others, such as phathalate and DDT, were not detected with the bacteria. These results suggest that endocrine disrupting chemicals are toxic in bacteria, and do not act via an estrogenic effect, and that toxicity monitoring and classification of some endocrine dis-rupting chemicals may be possible in the field using these freeze-dried recombinant bioluminescent bacteria.
AB - Five different freeze-dried recombinant bioluminescent bacteria were used for the detection of cellular stresses caused by endocrine disrupting chemicals. These strains were DPD2794 (recA::luxCDABE), which is sensitive to DNA damage, DPD2540 (fabA:: luxCDABE), sensitive to cellular membrane damage, DPD2511 (katG::luxCDABE), sensitive to oxidative damage, and TV1061 (grpE::luxCDABE), sensitive to protein damage. GC2, which emits bioluminescence constitutively, was also used in this study. The toxicity of several chemicals was determined on the first four freeze-dried bacteria, while nonspecific cellular stresses were measured using GC2. Damage caused by known endocrine disrupting chemicals, such as nonyl phenol, bisphenol A, and styrene, was detected and classified according to toxicity mode, while others, such as phathalate and DDT, were not detected with the bacteria. These results suggest that endocrine disrupting chemicals are toxic in bacteria, and do not act via an estrogenic effect, and that toxicity monitoring and classification of some endocrine dis-rupting chemicals may be possible in the field using these freeze-dried recombinant bioluminescent bacteria.
KW - Cellular toxicity
KW - Endocrine disrupting chemicals (EDCs)
KW - Freeze-dried recombinant bioluminescent bacteria
KW - Toxic effect
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U2 - 10.1007/BF02931937
DO - 10.1007/BF02931937
M3 - Article
AN - SCOPUS:7444224877
SN - 1226-8372
VL - 5
SP - 395
EP - 399
JO - Biotechnology and Bioprocess Engineering
JF - Biotechnology and Bioprocess Engineering
IS - 6
ER -