Toxicological evaluation of the isoflavone puerarin and its glycosides

Hyuk Jin Chung, Mi J. Chung, Soung J. Houng, Jungae Jeun, Dong Keon Kweon, Chung Hyo Choi, Jong T. Park, Kwan H. Park, Sung-Joon Lee

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Puerarin, an isoflavone derived from kudzu roots, has strong biological activities. However, its bioavailability in vivo is often limited by its insolubility. A novel transglycosylase increases the solubility of puerarin >100-fold, by converting it to puerarin glycosides. Since over-consumption of an isoflavone might have toxic effects, therefore, we investigated the potential antimutagenic activity, bone marrow micronucleus test, and a 28-day oral repeated administration test with puerarin and its glycosides. In Ames tests, neither puerarin nor its glycosides exhibited mutagenic effects up to 200 μg/plate. Puerarin and its glycoside, glucosyl-α-(1,6)-puerarin, significantly reduced the mutagenic effect of 4-nitroquinoline-1-oxide by up to 41%. In bone marrow micronucleus tests using ICR mice, neither puerarin nor glucosyl-α-(1,6)-puerarin interfered with erythrocyte production in the bone marrow. Both compounds decreased the prevalence of polychromatic erythrocytes. Sprague-Dawley rats were orally dosed with puerarin and its glycosides daily for 28 days. Neither puerarin nor its glycosides caused significant alterations in histology, and biochemical and hematologic parameters. These results suggest that puerarin and its glycosides do not have significant toxic effects, at least in rodents, either in vitro or in vivo at doses of up to 250 mg/kg per day.

Original languageEnglish
Pages (from-to)145-153
Number of pages9
JournalEuropean Food Research and Technology
Volume230
Issue number1
DOIs
Publication statusPublished - 2009 Oct 1

Fingerprint

Glycosides
Isoflavones
isoflavones
Toxicology
glycosides
bone marrow
Bone
erythrocytes
Solubility
nitroquinolines
Micronucleus Tests
Pueraria montana var. lobata
Poisons
Bone Marrow
Ames test
antimutagenic activity
Histology
testing
Bioactivity
puerarin

Keywords

  • Genotoxicity
  • Glycosylation
  • Puerarin
  • Twenty-eight-day oral repeated administration test

ASJC Scopus subject areas

  • Food Science
  • Biochemistry
  • Biotechnology
  • Chemistry(all)
  • Industrial and Manufacturing Engineering

Cite this

Toxicological evaluation of the isoflavone puerarin and its glycosides. / Chung, Hyuk Jin; Chung, Mi J.; Houng, Soung J.; Jeun, Jungae; Kweon, Dong Keon; Choi, Chung Hyo; Park, Jong T.; Park, Kwan H.; Lee, Sung-Joon.

In: European Food Research and Technology, Vol. 230, No. 1, 01.10.2009, p. 145-153.

Research output: Contribution to journalArticle

Chung, HJ, Chung, MJ, Houng, SJ, Jeun, J, Kweon, DK, Choi, CH, Park, JT, Park, KH & Lee, S-J 2009, 'Toxicological evaluation of the isoflavone puerarin and its glycosides', European Food Research and Technology, vol. 230, no. 1, pp. 145-153. https://doi.org/10.1007/s00217-009-1156-3
Chung, Hyuk Jin ; Chung, Mi J. ; Houng, Soung J. ; Jeun, Jungae ; Kweon, Dong Keon ; Choi, Chung Hyo ; Park, Jong T. ; Park, Kwan H. ; Lee, Sung-Joon. / Toxicological evaluation of the isoflavone puerarin and its glycosides. In: European Food Research and Technology. 2009 ; Vol. 230, No. 1. pp. 145-153.
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