Tracking and protection of transplanted stem cells using a ferrocenecarboxylic acid-conjugated peptide that mimics hTERT

Hyun Hee Park, Kyu Yong Lee, Dong Woo Park, Na Young Choi, Young Joo Lee, Jeong Woo Son, Sangjae Kim, Chanil Moon, Hyun Wook Kim, Im Joo Rhyu, Seong Ho Koh

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In vivo tracking of transplanted stem cells has been a central aim of stem cell therapy. Although many tracking systems have been introduced, no method has yet been validated for clinical applications. We developed a novel sophisticated peptide (GV1001) that mimics hTERT (human telomerase reverse transcriptase) and analysed its ability to track and protect stem cells after transplantation. Ferrocenecarboxylic acid-conjugated GV1001 (Fe-GV1001) efficiently penetrated stem cells with no adverse effects. Moreover, Fe-GV1001 improved the viability, proliferation, and migration of stem cells under hypoxia. After Fe-GV1001-labelled stem cells were transplanted into the brains of rats after stroke, the labelled cells were easily tracked by MRI. Our findings indicate that Fe-GV1001 can be used for the in vivo tracking of stem cells after transplantation into the brain and can improve the efficacy of stem cell therapy by sustaining and enhancing stem cell characteristics under disease conditions.

Original languageEnglish
Pages (from-to)80-91
Number of pages12
JournalBiomaterials
Volume155
DOIs
Publication statusPublished - 2018 Feb 1

Fingerprint

Stem cells
Peptides
Stem Cells
Acids
Stem Cell Transplantation
Cell- and Tissue-Based Therapy
Brain
Cell Hypoxia
human TERT protein
ferrocenecarboxylic acid
Stroke
Magnetic resonance imaging
Rats

Keywords

  • hTERT
  • Mesenchymal stem cells
  • MRI
  • Neural stem cells
  • Peptide
  • Tracking

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Tracking and protection of transplanted stem cells using a ferrocenecarboxylic acid-conjugated peptide that mimics hTERT. / Park, Hyun Hee; Lee, Kyu Yong; Park, Dong Woo; Choi, Na Young; Lee, Young Joo; Son, Jeong Woo; Kim, Sangjae; Moon, Chanil; Kim, Hyun Wook; Rhyu, Im Joo; Koh, Seong Ho.

In: Biomaterials, Vol. 155, 01.02.2018, p. 80-91.

Research output: Contribution to journalArticle

Park, Hyun Hee ; Lee, Kyu Yong ; Park, Dong Woo ; Choi, Na Young ; Lee, Young Joo ; Son, Jeong Woo ; Kim, Sangjae ; Moon, Chanil ; Kim, Hyun Wook ; Rhyu, Im Joo ; Koh, Seong Ho. / Tracking and protection of transplanted stem cells using a ferrocenecarboxylic acid-conjugated peptide that mimics hTERT. In: Biomaterials. 2018 ; Vol. 155. pp. 80-91.
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