Tracking of STAT3 signaling for anticancer drug-discovery based on localized surface plasmon resonance

Sojin Song, Anh H. Nguyen, Jong Uk Lee, Misun Cha, Sang Jun Sim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Signal transducer and activator of transcription 3 (STAT3) protein signaling is crucial for the survival, invasion, and growth of human cancer cells; thus, STAT3 protein is an ideal target for a new drug screening system. Herein, we developed a label-free sensor for anticancer drug-discovery based on the localized surface plasmon resonance (LSPR) shift response by tracking of STAT3 signaling including phosphorylation and dimerization. This enables ultrasensitive monitoring of the molecular interactions that occur on the surface of single gold nanoparticles. The red shift of the LSPR λmax was observed as 3.46 nm and 9.00 nm, respectively, indicating phosphorylation and dimerization of the STAT3 signaling pathway. In screening of anticancer candidates, the system worked well in the presence of STA-21 which inhibits STAT3 dimerization. The LSPR λmax shift in the inhibition condition is three times lower than that in the absence of an inhibitor. Interestingly, the system reveals high specificity, reproducibility and compatibility with real samples (MCF-7 cell line). Therefore, these results demonstrated that this system has strong potential to be an accurate and effective sensor for tracking of signaling pathways and drug screening of anticancer candidates for anticancer therapy.

Original languageEnglish
Pages (from-to)2493-2501
Number of pages9
JournalAnalyst
Volume141
Issue number8
DOIs
Publication statusPublished - 2016 Apr 21

Fingerprint

STAT3 Transcription Factor
Surface Plasmon Resonance
Surface plasmon resonance
Drug Discovery
Transcription
transducer
Transducers
drug
Dimerization
STAT Transcription Factors
Screening
Preclinical Drug Evaluations
Phosphorylation
Cells
sensor
Proteins
protein
Molecular interactions
MCF-7 Cells
Sensors

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Electrochemistry
  • Biochemistry
  • Environmental Chemistry

Cite this

Tracking of STAT3 signaling for anticancer drug-discovery based on localized surface plasmon resonance. / Song, Sojin; Nguyen, Anh H.; Lee, Jong Uk; Cha, Misun; Sim, Sang Jun.

In: Analyst, Vol. 141, No. 8, 21.04.2016, p. 2493-2501.

Research output: Contribution to journalArticle

Song, Sojin ; Nguyen, Anh H. ; Lee, Jong Uk ; Cha, Misun ; Sim, Sang Jun. / Tracking of STAT3 signaling for anticancer drug-discovery based on localized surface plasmon resonance. In: Analyst. 2016 ; Vol. 141, No. 8. pp. 2493-2501.
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