Traction microscopy with integrated microfluidics: Responses of the multi-cellular island to gradients of HGF

Hwanseok Jang, Jongseong Kim, Jennifer H. Shin, Jeffrey J. Fredberg, Chan Young Park, Yongdoo Park

Research output: Contribution to journalArticle

Abstract

Collective cellular migration plays a central role in development, regeneration, and metastasis. In these processes, mechanical interactions between cells are fundamental but measurement of these interactions is often hampered by technical limitations. To overcome some of these limitations, here we describe a system that integrates microfluidics with traction microscopy (TM). Using this system we can measure simultaneously, and in real time, migration speeds, tractions, and intercellular tension throughout an island of confluent Madin-Darby canine kidney (MDCK) cells. The cell island is exposed to hepatocyte growth factor (HGF) at a controlled gradient of concentrations; HGF is known to elicit epithelial-to-mesenchymal transition (EMT) and cell scattering. As expected, the rate of expansion of the cell island was dependent on the concentration of HGF. Higher concentrations of HGF reduced intercellular tensions, as expected during EMT. A novel finding, however, is that the effects of HGF concentration and its gradient were seen within an island. This integrated experimental system thus provides an integrated tool to better understand cellular forces during collective cellular migration under chemical gradients.

Original languageEnglish
Pages (from-to)1579-1588
Number of pages10
JournalLab on a Chip
Volume19
Issue number9
DOIs
Publication statusPublished - 2019 Jan 1

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Microfluidics
Hepatocyte Growth Factor
Traction
Islands
Microscopy
Microscopic examination
Epithelial-Mesenchymal Transition
Mechanical Phenomena
Madin Darby Canine Kidney Cells
Cell Communication
Regeneration
Intercellular Signaling Peptides and Proteins
Hepatocytes
Scattering
Neoplasm Metastasis

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Traction microscopy with integrated microfluidics : Responses of the multi-cellular island to gradients of HGF. / Jang, Hwanseok; Kim, Jongseong; Shin, Jennifer H.; Fredberg, Jeffrey J.; Park, Chan Young; Park, Yongdoo.

In: Lab on a Chip, Vol. 19, No. 9, 01.01.2019, p. 1579-1588.

Research output: Contribution to journalArticle

Jang, Hwanseok ; Kim, Jongseong ; Shin, Jennifer H. ; Fredberg, Jeffrey J. ; Park, Chan Young ; Park, Yongdoo. / Traction microscopy with integrated microfluidics : Responses of the multi-cellular island to gradients of HGF. In: Lab on a Chip. 2019 ; Vol. 19, No. 9. pp. 1579-1588.
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