TY - JOUR
T1 - Traction microscopy with integrated microfluidics
T2 - Responses of the multi-cellular island to gradients of HGF
AU - Jang, Hwanseok
AU - Kim, Jongseong
AU - Shin, Jennifer H.
AU - Fredberg, Jeffrey J.
AU - Park, Chan Young
AU - Park, Yongdoo
N1 - Funding Information:
We thank numerous colleagues who provided insightful help with the preparation in this MS. Special thanks to Prof. H. Chun at Korea University, Dr. B. Gweon at Hanyang University, and Mr. Y. Cho at KAIST for the supply of experimental materials. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. NRF-2017R1E1A1A01075103), Korea University Grant, and the BK 21 Plus program.
Publisher Copyright:
© The Royal Society of Chemistry.
PY - 2019/5/7
Y1 - 2019/5/7
N2 - Collective cellular migration plays a central role in development, regeneration, and metastasis. In these processes, mechanical interactions between cells are fundamental but measurement of these interactions is often hampered by technical limitations. To overcome some of these limitations, here we describe a system that integrates microfluidics with traction microscopy (TM). Using this system we can measure simultaneously, and in real time, migration speeds, tractions, and intercellular tension throughout an island of confluent Madin-Darby canine kidney (MDCK) cells. The cell island is exposed to hepatocyte growth factor (HGF) at a controlled gradient of concentrations; HGF is known to elicit epithelial-to-mesenchymal transition (EMT) and cell scattering. As expected, the rate of expansion of the cell island was dependent on the concentration of HGF. Higher concentrations of HGF reduced intercellular tensions, as expected during EMT. A novel finding, however, is that the effects of HGF concentration and its gradient were seen within an island. This integrated experimental system thus provides an integrated tool to better understand cellular forces during collective cellular migration under chemical gradients.
AB - Collective cellular migration plays a central role in development, regeneration, and metastasis. In these processes, mechanical interactions between cells are fundamental but measurement of these interactions is often hampered by technical limitations. To overcome some of these limitations, here we describe a system that integrates microfluidics with traction microscopy (TM). Using this system we can measure simultaneously, and in real time, migration speeds, tractions, and intercellular tension throughout an island of confluent Madin-Darby canine kidney (MDCK) cells. The cell island is exposed to hepatocyte growth factor (HGF) at a controlled gradient of concentrations; HGF is known to elicit epithelial-to-mesenchymal transition (EMT) and cell scattering. As expected, the rate of expansion of the cell island was dependent on the concentration of HGF. Higher concentrations of HGF reduced intercellular tensions, as expected during EMT. A novel finding, however, is that the effects of HGF concentration and its gradient were seen within an island. This integrated experimental system thus provides an integrated tool to better understand cellular forces during collective cellular migration under chemical gradients.
UR - http://www.scopus.com/inward/record.url?scp=85064972064&partnerID=8YFLogxK
U2 - 10.1039/c9lc00173e
DO - 10.1039/c9lc00173e
M3 - Article
C2 - 30924490
AN - SCOPUS:85064972064
SN - 1473-0197
VL - 19
SP - 1579
EP - 1588
JO - Lab on a Chip - Miniaturisation for Chemistry and Biology
JF - Lab on a Chip - Miniaturisation for Chemistry and Biology
IS - 9
ER -