Transcription factor GATA-3 regulates the transcriptional activity of dopamine β-hydroxylase by interacting with Sp1 and AP4

Seok Jong Hong, Hyun Jin Choi, Sunghoi Hong, Youngbuhm Huh, Han Chae, Kwang Soo Kim

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

GATA-3 is a zinc finger transcription factor that is expressed in T cell lineages as well as in the nervous system during development. In this study, we report that forced expression of GATA-3 resulted in an increased number of dopamine β-hydroxylase (DBH)-expressing neurons in primary neural crest stem cell (NCSC) culture, suggesting that the DBH gene may be a downstream target gene of GATA-3. GATA-3 robustly transactivates the promoter function of the noradrenaline (NA)-synthesizing DBH gene, via two specific upstream promoter domains; one at -62 to -32 bp and the other at -891 to -853 bp. Surprisingly, none of these domains contain GATA-3 binding sites but encompass binding motifs for transcription factors Sp1 and AP4, respectively. Protein-protein interaction analyses both in vitro and in vivo and chromatin immunoprecipitation (ChIP) assays showed that GATA-3 effects its transcriptional regulatory function through physical interactions with these transcription factors.

Original languageEnglish
Pages (from-to)1821-1831
Number of pages11
JournalNeurochemical Research
Volume33
Issue number9
DOIs
Publication statusPublished - 2008 Sep 1
Externally publishedYes

Fingerprint

GATA3 Transcription Factor
Transcription Factor 3
GATA Transcription Factors
Mixed Function Oxygenases
Dopamine
Genes
Transcription Factors
Sp1 Transcription Factor
T-cells
Neural Stem Cells
Neural Crest
Chromatin Immunoprecipitation
Zinc Fingers
Neurology
Cell Lineage
Stem cells
Cell culture
Nervous System
Neurons
Chromatin

Keywords

  • AP4
  • ChIP assay
  • Dopamine β-hydroxylase
  • GATA-3
  • Protein-protein interaction
  • siRNA
  • Sp1

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

Transcription factor GATA-3 regulates the transcriptional activity of dopamine β-hydroxylase by interacting with Sp1 and AP4. / Hong, Seok Jong; Choi, Hyun Jin; Hong, Sunghoi; Huh, Youngbuhm; Chae, Han; Kim, Kwang Soo.

In: Neurochemical Research, Vol. 33, No. 9, 01.09.2008, p. 1821-1831.

Research output: Contribution to journalArticle

Hong, Seok Jong ; Choi, Hyun Jin ; Hong, Sunghoi ; Huh, Youngbuhm ; Chae, Han ; Kim, Kwang Soo. / Transcription factor GATA-3 regulates the transcriptional activity of dopamine β-hydroxylase by interacting with Sp1 and AP4. In: Neurochemical Research. 2008 ; Vol. 33, No. 9. pp. 1821-1831.
@article{fda1f78502a84b11807ff4093da048fb,
title = "Transcription factor GATA-3 regulates the transcriptional activity of dopamine β-hydroxylase by interacting with Sp1 and AP4",
abstract = "GATA-3 is a zinc finger transcription factor that is expressed in T cell lineages as well as in the nervous system during development. In this study, we report that forced expression of GATA-3 resulted in an increased number of dopamine β-hydroxylase (DBH)-expressing neurons in primary neural crest stem cell (NCSC) culture, suggesting that the DBH gene may be a downstream target gene of GATA-3. GATA-3 robustly transactivates the promoter function of the noradrenaline (NA)-synthesizing DBH gene, via two specific upstream promoter domains; one at -62 to -32 bp and the other at -891 to -853 bp. Surprisingly, none of these domains contain GATA-3 binding sites but encompass binding motifs for transcription factors Sp1 and AP4, respectively. Protein-protein interaction analyses both in vitro and in vivo and chromatin immunoprecipitation (ChIP) assays showed that GATA-3 effects its transcriptional regulatory function through physical interactions with these transcription factors.",
keywords = "AP4, ChIP assay, Dopamine β-hydroxylase, GATA-3, Protein-protein interaction, siRNA, Sp1",
author = "Hong, {Seok Jong} and Choi, {Hyun Jin} and Sunghoi Hong and Youngbuhm Huh and Han Chae and Kim, {Kwang Soo}",
year = "2008",
month = "9",
day = "1",
doi = "10.1007/s11064-008-9639-3",
language = "English",
volume = "33",
pages = "1821--1831",
journal = "Neurochemical Research",
issn = "0364-3190",
publisher = "Springer New York",
number = "9",

}

TY - JOUR

T1 - Transcription factor GATA-3 regulates the transcriptional activity of dopamine β-hydroxylase by interacting with Sp1 and AP4

AU - Hong, Seok Jong

AU - Choi, Hyun Jin

AU - Hong, Sunghoi

AU - Huh, Youngbuhm

AU - Chae, Han

AU - Kim, Kwang Soo

PY - 2008/9/1

Y1 - 2008/9/1

N2 - GATA-3 is a zinc finger transcription factor that is expressed in T cell lineages as well as in the nervous system during development. In this study, we report that forced expression of GATA-3 resulted in an increased number of dopamine β-hydroxylase (DBH)-expressing neurons in primary neural crest stem cell (NCSC) culture, suggesting that the DBH gene may be a downstream target gene of GATA-3. GATA-3 robustly transactivates the promoter function of the noradrenaline (NA)-synthesizing DBH gene, via two specific upstream promoter domains; one at -62 to -32 bp and the other at -891 to -853 bp. Surprisingly, none of these domains contain GATA-3 binding sites but encompass binding motifs for transcription factors Sp1 and AP4, respectively. Protein-protein interaction analyses both in vitro and in vivo and chromatin immunoprecipitation (ChIP) assays showed that GATA-3 effects its transcriptional regulatory function through physical interactions with these transcription factors.

AB - GATA-3 is a zinc finger transcription factor that is expressed in T cell lineages as well as in the nervous system during development. In this study, we report that forced expression of GATA-3 resulted in an increased number of dopamine β-hydroxylase (DBH)-expressing neurons in primary neural crest stem cell (NCSC) culture, suggesting that the DBH gene may be a downstream target gene of GATA-3. GATA-3 robustly transactivates the promoter function of the noradrenaline (NA)-synthesizing DBH gene, via two specific upstream promoter domains; one at -62 to -32 bp and the other at -891 to -853 bp. Surprisingly, none of these domains contain GATA-3 binding sites but encompass binding motifs for transcription factors Sp1 and AP4, respectively. Protein-protein interaction analyses both in vitro and in vivo and chromatin immunoprecipitation (ChIP) assays showed that GATA-3 effects its transcriptional regulatory function through physical interactions with these transcription factors.

KW - AP4

KW - ChIP assay

KW - Dopamine β-hydroxylase

KW - GATA-3

KW - Protein-protein interaction

KW - siRNA

KW - Sp1

UR - http://www.scopus.com/inward/record.url?scp=48349146738&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=48349146738&partnerID=8YFLogxK

U2 - 10.1007/s11064-008-9639-3

DO - 10.1007/s11064-008-9639-3

M3 - Article

C2 - 18338249

AN - SCOPUS:48349146738

VL - 33

SP - 1821

EP - 1831

JO - Neurochemical Research

JF - Neurochemical Research

SN - 0364-3190

IS - 9

ER -