Transcription of Corynebacterium glutamicum genes involved in tricarboxylic acid cycle and glyoxylate cycle

Sung Ok Han, Masayuki Inui, Hideaki Yukawa

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Transcription of the tricarboxylic acid cycle genes of Corynebacterium glutamicum was investigated. Northern hybridizations revealed that gltA-fkb, odhA-orfA, sucC-sucD, sdhC-sdhA-sdhB-orfB and mdh-orfC were transcribed as polycistronic mRNAs of size 1.9, 4.5, 2.5, 4.0 and 1.7 kb, respectively. The acn-acnR-gat gene cluster was transcribed as a mono-, bi- or tricistronic mRNA, depending on the carbon source. The 2.9-kb (acn) and 1.5-kb (acnR-gat) mRNAs, which were regulated by different promoters upstream of acn and acnR, were inversely expressed in acetate and glucose. The 4.5 kb (acn-acnR-gat) mRNA was constitutively expressed. The sizes of the mRNAs were 2.3, 2.1, 1.5, 1.3, 1.7, 1.5 and 2.9 kb for icd, sucB, fum, mdhB, mqo, aceA and aceB, respectively, indicating monocistronic transcription of these genes. RNA ligase-mediated rapid amplification of cDNA ends analysis of C. glutamicum RNA showed that the transcriptional start sites of gltA, acn, icd, odhA, sucB, sucC, sdhC, fum, mdh, mdhB, mqo, aceA and aceB were located 121, 107, 31, 99, 46, 83, 15, 25, 33, 23, 70, 111 and 183 bp upstream from the first nucleotide of the respective translation initiation codons. Alignment of these gene promoter regions provided evidence for highly conserved sequences that exhibited similarity to the σA consensus promoter sequences of Gram-positive bacteria.

Original languageEnglish
Pages (from-to)264-276
Number of pages13
JournalJournal of Molecular Microbiology and Biotechnology
Volume15
Issue number4
DOIs
Publication statusPublished - 2008 Sep 1

Fingerprint

Corynebacterium glutamicum
Citric Acid Cycle
Transcription
Genes
Messenger RNA
RNA
Initiator Codon
Conserved Sequence
Consensus Sequence
Gram-Positive Bacteria
Ligases
Multigene Family
Nucleotides
Genetic Promoter Regions
Amplification
Glucose
Bacteria
Acetates
Carbon
Complementary DNA

Keywords

  • Corynebacterium glutamicum
  • Promoter
  • Transcription
  • Tricarboxylic acid cycle

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Transcription of Corynebacterium glutamicum genes involved in tricarboxylic acid cycle and glyoxylate cycle. / Han, Sung Ok; Inui, Masayuki; Yukawa, Hideaki.

In: Journal of Molecular Microbiology and Biotechnology, Vol. 15, No. 4, 01.09.2008, p. 264-276.

Research output: Contribution to journalArticle

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