Transcriptional analysis of genes encoding β-glucosidase of Schizophyllum commune KUC9397 under optimal conditions

Young Min Lee, Hanbyul Lee, Young Mok Heo, Hwanhwi Lee, Joo Hyun Hong, Jae-Jin Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The present study was conducted to determine the gene responsible for beta-glucosidase (BGL) production and to generate a full-length complementary DNA (cDNA) of one of the putative BGL genes, which showed a significant expression level when Schizophyllum commune KUC9397 was grown in optimized medium. The relative expression levels of seven genes encoding BGL of S. commune KUC9397 were determined with real-time quantitative reverse transcription PCR in cellulose-containing optimized medium (OM) compared to glucose-containing basal medium (BM). The most abundant transcript was bgl3a in OM. The transcript number of the bgl3a increased more than 57.60-fold when S. commune KUC9397 was grown on cellulose-containing OM compared to that on glucose-containing BM. The bgl3a was identified, and a deduced amino acid sequence of bgl3a shared homology (97%) with GH3 BGL of S. commune H4-8. This is the first report showing the transcription levels of genes encoding BGL and identification of full-length cDNA of glycoside hydrolase 3 (GH3) BGL from S. commune. Furthermore, this study is one of the steps for consolidated bioprocessing of lignocellulosic biomass to bioethanol.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalFolia Microbiologica
DOIs
Publication statusAccepted/In press - 2016 Nov 30

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Schizophyllum
Glucosidases
beta-Glucosidase
Genes
Glycoside Hydrolases
Cellulose
Complementary DNA
Glucose
Biomass
Reverse Transcription
Amino Acid Sequence
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Microbiology

Cite this

Transcriptional analysis of genes encoding β-glucosidase of Schizophyllum commune KUC9397 under optimal conditions. / Lee, Young Min; Lee, Hanbyul; Heo, Young Mok; Lee, Hwanhwi; Hong, Joo Hyun; Kim, Jae-Jin.

In: Folia Microbiologica, 30.11.2016, p. 1-6.

Research output: Contribution to journalArticle

Lee, Young Min ; Lee, Hanbyul ; Heo, Young Mok ; Lee, Hwanhwi ; Hong, Joo Hyun ; Kim, Jae-Jin. / Transcriptional analysis of genes encoding β-glucosidase of Schizophyllum commune KUC9397 under optimal conditions. In: Folia Microbiologica. 2016 ; pp. 1-6.
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