Transcriptional control of the RECK metastasis/angiogenesis suppressor gene

Regina Maki Sasahara, Sheila Maria Brochado, Chiaki Takahashi, Junseo Oh, Silvya Stuchi Maria-Engler, José Mauro Granjeiro, Makoto Noda, Mari Cleide Sogayar

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The RECK gene is widely expressed in normal human tissues but is downregulated in tumor cell lines and oncogenically transformed fibroblasts. RECK encodes a membrane-anchored glycoprotein that suppresses tumor invasion and angiogenesis by regulating matrix-metalloproteinases (MMP-2, MMP-9 and MT1-MMP). Understanding of the transcriptional regulation of tumor/metastasis suppressor genes constitutes a potent approach to the molecular basis of malignant transformation. In order to uncover the mechanisms of control of RECK gene expression, the RECK promoter has been cloned and characterized. One of the elements responsible for the Ras-mediated downregulation of mouse RECK gene is the Sp1 site, to which Sp1 and Sp3 factors bind. Other regulatory events, such as DNA methylation of the RECK promoter and histone acetylation/deacetylation have been studied to understand the underlying mechanisms of RECK expression. Understanding of the mechanisms which control RECK gene transcription may lead to the development of new strategies for cancer prevention and treatment.

Original languageEnglish
Pages (from-to)435-443
Number of pages9
JournalCancer Detection and Prevention
Volume26
Issue number6
DOIs
Publication statusPublished - 2002
Externally publishedYes

Keywords

  • Angiogenesis
  • Metastasis
  • Sp1 transcription factor
  • Transcriptional control
  • Tumor suppressor gene

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Sasahara, R. M., Brochado, S. M., Takahashi, C., Oh, J., Maria-Engler, S. S., Granjeiro, J. M., Noda, M., & Sogayar, M. C. (2002). Transcriptional control of the RECK metastasis/angiogenesis suppressor gene. Cancer Detection and Prevention, 26(6), 435-443. https://doi.org/10.1016/S0361-090X(02)00123-X