Transcriptional regulation of selenoprotein W by MyoD during early skeletal muscle differentiation

Ok Jeong Noh, Yong Hwan Park, Youn Wook Chung, Ick Young Kim

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Selenoprotein W (SelW) is expressed in various tissues, but it is especially high in the skeletal muscle of mammals. Such tissue-specific protein expression implies regulation by a tissue-specific factor. In this study, we investigated SelW expression during myogenic C2C12 cell differentiation using RTPCR, quantitative PCR, and Western blot analysis. Both the protein and mRNA levels of SelW were increased during C2C12 cell differentiation, particularly during the early stage. Sequence analysis of the SelW promoter revealed four putative E-boxes, E1, E2, E3, and E4, which are known binding sites for MyoD, a myogenic transcriptional factor. Luciferase reporter assay showed that E1 and E4 were crucial for MyoD-dependent promoter activity. Using EMSA analysis, we observed that MyoD bound directly to E1 but not to E4, even though E4 mutation reduced SelW promoter activity in the luciferase reporter assay. Binding of MyoD to E1 was further investigated by ChIP assay. These results suggest that the SelW gene was activated by the binding of MyoD to a specific E-box during early skeletal muscle differentiation.

Original languageEnglish
Pages (from-to)40496-40507
Number of pages12
JournalJournal of Biological Chemistry
Volume285
Issue number52
DOIs
Publication statusPublished - 2010 Dec 24

Fingerprint

Selenoprotein W
Muscle
Skeletal Muscle
Assays
Tissue
Luciferases
Cell Differentiation
Mammals
Thromboplastin
Sequence Analysis
Proteins
Genes
Western Blotting
Binding Sites
Polymerase Chain Reaction
Messenger RNA
Mutation

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Transcriptional regulation of selenoprotein W by MyoD during early skeletal muscle differentiation. / Noh, Ok Jeong; Park, Yong Hwan; Chung, Youn Wook; Kim, Ick Young.

In: Journal of Biological Chemistry, Vol. 285, No. 52, 24.12.2010, p. 40496-40507.

Research output: Contribution to journalArticle

Noh, Ok Jeong ; Park, Yong Hwan ; Chung, Youn Wook ; Kim, Ick Young. / Transcriptional regulation of selenoprotein W by MyoD during early skeletal muscle differentiation. In: Journal of Biological Chemistry. 2010 ; Vol. 285, No. 52. pp. 40496-40507.
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