Transcriptional repression of type I procollagen genes during adipocyte differentiation

Tacghee Yi, Hee Man Choi, Rang Woon Park, Kun Young Sohn, In San Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Adipocyte differentiation is a very complex process in which whole-cell changes are accompanied. Among them, type I procollagen gene has been shown to specifically decrease during adipocyte differentiation; however, little is known about the molecular mechanism. To examine how type I procollagen gene expression is regulated at the level of transcription during adipocyte differentiation, 3T3-L1 preadipocyte cell line was used as an in vitro model. Northern blot analysis demonstrated that mRNA expression of type I procollagen gene was dramatically reduced during adipocyte differentiation. Time-course analysis indicated that decrease in mRNA expression occurred at early stage of differentiation. Studies on several stable cell lines showed that transcriptional activities of both α1 and α2 promoters decreased significantly during adipocyte differentiation. Despite extensive deletion-promoter analyses, however, we could not identify the cis-element responsible for the switch-off of type I procollagen gene during adipocyte differentiation, suggesting that the transcriptional repression of this gene occur through general transcription machinery rather than a specific cis-element. In conclusion, down-regulation of type I procollagen mRNA expression during adipocyte differentiation is due to repression of its promoter activity through general transcription machinery.

Original languageEnglish
Pages (from-to)269-275
Number of pages7
JournalExperimental and Molecular Medicine
Volume33
Issue number4
DOIs
Publication statusPublished - 2001 Dec 31
Externally publishedYes

Keywords

  • Adipocyte differentiation
  • Procollagen
  • Repression
  • Transcription

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

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