Transcriptional repression of type I procollagen genes during adipocyte differentiation

Tacghee Yi, Hee Man Choi, Rang Woon Park, Kun Young Sohn, In-San Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Adipocyte differentiation is a very complex process in which whole-cell changes are accompanied. Among them, type I procollagen gene has been shown to specifically decrease during adipocyte differentiation; however, little is known about the molecular mechanism. To examine how type I procollagen gene expression is regulated at the level of transcription during adipocyte differentiation, 3T3-L1 preadipocyte cell line was used as an in vitro model. Northern blot analysis demonstrated that mRNA expression of type I procollagen gene was dramatically reduced during adipocyte differentiation. Time-course analysis indicated that decrease in mRNA expression occurred at early stage of differentiation. Studies on several stable cell lines showed that transcriptional activities of both α1 and α2 promoters decreased significantly during adipocyte differentiation. Despite extensive deletion-promoter analyses, however, we could not identify the cis-element responsible for the switch-off of type I procollagen gene during adipocyte differentiation, suggesting that the transcriptional repression of this gene occur through general transcription machinery rather than a specific cis-element. In conclusion, down-regulation of type I procollagen mRNA expression during adipocyte differentiation is due to repression of its promoter activity through general transcription machinery.

Original languageEnglish
Pages (from-to)269-275
Number of pages7
JournalExperimental and Molecular Medicine
Volume33
Issue number4
Publication statusPublished - 2001 Dec 31
Externally publishedYes

Fingerprint

Collagen Type I
Adipocytes
Genes
Transcription
Messenger RNA
Machinery
Cells
3T3-L1 Cells
Gene expression
Cell Line
Switches
Northern Blotting
Down-Regulation
Gene Expression

Keywords

  • Adipocyte differentiation
  • Procollagen
  • Repression
  • Transcription

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

Transcriptional repression of type I procollagen genes during adipocyte differentiation. / Yi, Tacghee; Choi, Hee Man; Park, Rang Woon; Sohn, Kun Young; Kim, In-San.

In: Experimental and Molecular Medicine, Vol. 33, No. 4, 31.12.2001, p. 269-275.

Research output: Contribution to journalArticle

Yi, Tacghee ; Choi, Hee Man ; Park, Rang Woon ; Sohn, Kun Young ; Kim, In-San. / Transcriptional repression of type I procollagen genes during adipocyte differentiation. In: Experimental and Molecular Medicine. 2001 ; Vol. 33, No. 4. pp. 269-275.
@article{f6ca9e8f0aa643d1b68235f65b28f939,
title = "Transcriptional repression of type I procollagen genes during adipocyte differentiation",
abstract = "Adipocyte differentiation is a very complex process in which whole-cell changes are accompanied. Among them, type I procollagen gene has been shown to specifically decrease during adipocyte differentiation; however, little is known about the molecular mechanism. To examine how type I procollagen gene expression is regulated at the level of transcription during adipocyte differentiation, 3T3-L1 preadipocyte cell line was used as an in vitro model. Northern blot analysis demonstrated that mRNA expression of type I procollagen gene was dramatically reduced during adipocyte differentiation. Time-course analysis indicated that decrease in mRNA expression occurred at early stage of differentiation. Studies on several stable cell lines showed that transcriptional activities of both α1 and α2 promoters decreased significantly during adipocyte differentiation. Despite extensive deletion-promoter analyses, however, we could not identify the cis-element responsible for the switch-off of type I procollagen gene during adipocyte differentiation, suggesting that the transcriptional repression of this gene occur through general transcription machinery rather than a specific cis-element. In conclusion, down-regulation of type I procollagen mRNA expression during adipocyte differentiation is due to repression of its promoter activity through general transcription machinery.",
keywords = "Adipocyte differentiation, Procollagen, Repression, Transcription",
author = "Tacghee Yi and Choi, {Hee Man} and Park, {Rang Woon} and Sohn, {Kun Young} and In-San Kim",
year = "2001",
month = "12",
day = "31",
language = "English",
volume = "33",
pages = "269--275",
journal = "Experimental and Molecular Medicine",
issn = "1226-3613",
publisher = "Korean Society of Med. Biochemistry and Mol. Biology",
number = "4",

}

TY - JOUR

T1 - Transcriptional repression of type I procollagen genes during adipocyte differentiation

AU - Yi, Tacghee

AU - Choi, Hee Man

AU - Park, Rang Woon

AU - Sohn, Kun Young

AU - Kim, In-San

PY - 2001/12/31

Y1 - 2001/12/31

N2 - Adipocyte differentiation is a very complex process in which whole-cell changes are accompanied. Among them, type I procollagen gene has been shown to specifically decrease during adipocyte differentiation; however, little is known about the molecular mechanism. To examine how type I procollagen gene expression is regulated at the level of transcription during adipocyte differentiation, 3T3-L1 preadipocyte cell line was used as an in vitro model. Northern blot analysis demonstrated that mRNA expression of type I procollagen gene was dramatically reduced during adipocyte differentiation. Time-course analysis indicated that decrease in mRNA expression occurred at early stage of differentiation. Studies on several stable cell lines showed that transcriptional activities of both α1 and α2 promoters decreased significantly during adipocyte differentiation. Despite extensive deletion-promoter analyses, however, we could not identify the cis-element responsible for the switch-off of type I procollagen gene during adipocyte differentiation, suggesting that the transcriptional repression of this gene occur through general transcription machinery rather than a specific cis-element. In conclusion, down-regulation of type I procollagen mRNA expression during adipocyte differentiation is due to repression of its promoter activity through general transcription machinery.

AB - Adipocyte differentiation is a very complex process in which whole-cell changes are accompanied. Among them, type I procollagen gene has been shown to specifically decrease during adipocyte differentiation; however, little is known about the molecular mechanism. To examine how type I procollagen gene expression is regulated at the level of transcription during adipocyte differentiation, 3T3-L1 preadipocyte cell line was used as an in vitro model. Northern blot analysis demonstrated that mRNA expression of type I procollagen gene was dramatically reduced during adipocyte differentiation. Time-course analysis indicated that decrease in mRNA expression occurred at early stage of differentiation. Studies on several stable cell lines showed that transcriptional activities of both α1 and α2 promoters decreased significantly during adipocyte differentiation. Despite extensive deletion-promoter analyses, however, we could not identify the cis-element responsible for the switch-off of type I procollagen gene during adipocyte differentiation, suggesting that the transcriptional repression of this gene occur through general transcription machinery rather than a specific cis-element. In conclusion, down-regulation of type I procollagen mRNA expression during adipocyte differentiation is due to repression of its promoter activity through general transcription machinery.

KW - Adipocyte differentiation

KW - Procollagen

KW - Repression

KW - Transcription

UR - http://www.scopus.com/inward/record.url?scp=0035980903&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035980903&partnerID=8YFLogxK

M3 - Article

VL - 33

SP - 269

EP - 275

JO - Experimental and Molecular Medicine

JF - Experimental and Molecular Medicine

SN - 1226-3613

IS - 4

ER -