Transcriptional response according to strength of calorie restriction in Saccharomyces cerevisiae

Yae Lim Lee, Cheol Koo Lee

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

To characterize gene expression that is dependent on the strength of calorie restriction (CR), we obtained transcriptome at different levels of glucose, which is a major energy and carbon source for budding yeast. To faithfully mimic mammalian CR in yeast culture, we reconstituted and grew seeding yeast cells in fresh 2% YPD media before inoculating into 2%, 1%, 0.5% and 0.25% YPD media to reflect different CR strengths. We collected and characterized 160 genes that responded to CR strength based on the rigorous statistical analyses of multiple test corrected ANOVA (adjusted p value < 0.1 or raw p value < 0.0031) and Pearson correlation ( r > 0.7). Based on the individual gene studies and the GO Term Finder analysis of 160 genes, we found that CR dose-dependently and gradually increased mitochondrial function at the transcriptional level. Therefore, we suggest these 160 genes are markers that respond to CR strength and that might be useful in elucidating CR mechanisms, especially how stronger CR extends life span more.

Original languageEnglish
Pages (from-to)299-307
Number of pages9
JournalMolecules and cells
Volume26
Issue number3
Publication statusPublished - 2008 Sep 30

Keywords

  • Calorie restriction
  • Glucose level
  • Life span extension
  • Mitochondria
  • Saccharomyces cerevisiae
  • Transcriptome

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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