Transcriptome analysis of acetic-acid-treated yeast cells identifies a large set of genes whose overexpression or deletion enhances acetic acid tolerance

Yeji Lee, Olviyani Nasution, Eunyong Choi, In-Geol Choi, Wankee Kim, Wonja Choi

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Acetic acid inhibits the metabolic activities of Saccharomyces cerevisiae. Therefore, a better understanding of how S. cerevisiae cells acquire the tolerance to acetic acid is of importance to develop robust yeast strains to be used in industry. To do this, we examined the transcriptional changes that occur at 12 h post-exposure to acetic acid, revealing that 56 and 58 genes were upregulated and downregulated, respectively. Functional categorization of them revealed that 22 protein synthesis genes and 14 stress response genes constituted the largest portion of the upregulated and downregulated genes, respectively. To evaluate the association of the regulated genes with acetic acid tolerance, 3 upregulated genes (DBP2, ASC1, and GND1) were selected among 34 non-protein synthesis genes, and 54 viable mutants individually deleted for the downregulated genes were retrieved from the non-essential haploid deletion library. Strains overexpressing ASC1 and GND1 displayed enhanced tolerance to acetic acid, whereas a strain overexpressing DBP2 was sensitive. Fifty of 54 deletion mutants displayed enhanced acetic acid tolerance. Three chosen deletion mutants (hsps82Δ, ato2Δ, and ssa3Δ) were also tolerant to benzoic acid but not propionic and sorbic acids. Moreover, all those five (two overexpressing and three deleted) strains were more efficient in proton efflux and lower in membrane permeability and internal hydrogen peroxide content than controls. Individually or in combination, those physiological changes are likely to contribute at least in part to enhanced acetic acid tolerance. Overall, information of our transcriptional profile was very useful to identify molecular factors associated with acetic acid tolerance.

Original languageEnglish
Pages (from-to)6391-6403
Number of pages13
JournalApplied Microbiology and Biotechnology
Volume99
Issue number15
DOIs
Publication statusPublished - 2015 Jun 11

Fingerprint

Gene Expression Profiling
Acetic Acid
Yeasts
Genes
Down-Regulation
Saccharomyces cerevisiae
Benzoic Acid
Haploidy
Propionates
Hydrogen Peroxide
Libraries
Protons
Permeability
Industry
Membranes

Keywords

  • Acetic acid
  • Deletion mutant
  • Overexpression
  • Stress tolerance
  • Transcriptome profile

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Transcriptome analysis of acetic-acid-treated yeast cells identifies a large set of genes whose overexpression or deletion enhances acetic acid tolerance. / Lee, Yeji; Nasution, Olviyani; Choi, Eunyong; Choi, In-Geol; Kim, Wankee; Choi, Wonja.

In: Applied Microbiology and Biotechnology, Vol. 99, No. 15, 11.06.2015, p. 6391-6403.

Research output: Contribution to journalArticle

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