Transcriptome analysis of Aspergillus fumigatus exposed to voriconazole

Márcia Eliana da Silva Ferreira, Iran Malavazi, Marcela Savoldi, Axel A. Brakhage, Maria Helena S Goldman, Heenam Kim, William C. Nierman, Gustavo H. Goldman

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

For a comprehensive evaluation of genes that have their expression modulated during exposure of the mycelia to voriconazole, we performed a large-scale analysis of gene expression in Aspergillus fumigatus using a microarray hybridization approach. By comparing the expression of genes between the reference time and after addition of voriconazole (30, 60, 120, and 240 min), we identified 2,271 genes differentially expressed in the wild-type strain. To validate the expression of some of these genes during exposure to voriconazole, we analyzed 13 genes showing higher expression in the presence of voriconazole by real-time RT-PCR. Although the magnitudes of induction differed between the two experimental systems, in about 85% of the cases they were in good agreement with the microarray data. To our knowledge this is the first study of microarray hybridization analysis for a filamentous fungus exposed to an antifungal agent. In our study, we have observed: (i) a decreased mRNA expression of various ergosterol biosynthesis genes; (ii) increased mRNA levels of genes involved in a variety of cell functions, such as transporters, transcription factors, proteins involved in cell metabolism, and hypothetical proteins; and (iii) the involvement of the cyclic AMP-protein kinase signaling pathway in the increased mRNA expression of several of these genes.

Original languageEnglish
Pages (from-to)32-44
Number of pages13
JournalCurrent Genetics
Volume50
Issue number1
DOIs
Publication statusPublished - 2006 Jul 1
Externally publishedYes

Fingerprint

Aspergillus fumigatus
Gene Expression Profiling
Gene Expression
Genes
Messenger RNA
Ergosterol
Adenylate Kinase
Antifungal Agents
Mycelium
Microarray Analysis
Cyclic AMP
Protein Kinases
Real-Time Polymerase Chain Reaction
Proteins
Fungi
Transcription Factors
Voriconazole

Keywords

  • Aspergillus fumigatus
  • Microarrays
  • Transcriptome
  • Voriconazole

ASJC Scopus subject areas

  • Genetics

Cite this

da Silva Ferreira, M. E., Malavazi, I., Savoldi, M., Brakhage, A. A., Goldman, M. H. S., Kim, H., ... Goldman, G. H. (2006). Transcriptome analysis of Aspergillus fumigatus exposed to voriconazole. Current Genetics, 50(1), 32-44. https://doi.org/10.1007/s00294-006-0073-2

Transcriptome analysis of Aspergillus fumigatus exposed to voriconazole. / da Silva Ferreira, Márcia Eliana; Malavazi, Iran; Savoldi, Marcela; Brakhage, Axel A.; Goldman, Maria Helena S; Kim, Heenam; Nierman, William C.; Goldman, Gustavo H.

In: Current Genetics, Vol. 50, No. 1, 01.07.2006, p. 32-44.

Research output: Contribution to journalArticle

da Silva Ferreira, ME, Malavazi, I, Savoldi, M, Brakhage, AA, Goldman, MHS, Kim, H, Nierman, WC & Goldman, GH 2006, 'Transcriptome analysis of Aspergillus fumigatus exposed to voriconazole', Current Genetics, vol. 50, no. 1, pp. 32-44. https://doi.org/10.1007/s00294-006-0073-2
da Silva Ferreira ME, Malavazi I, Savoldi M, Brakhage AA, Goldman MHS, Kim H et al. Transcriptome analysis of Aspergillus fumigatus exposed to voriconazole. Current Genetics. 2006 Jul 1;50(1):32-44. https://doi.org/10.1007/s00294-006-0073-2
da Silva Ferreira, Márcia Eliana ; Malavazi, Iran ; Savoldi, Marcela ; Brakhage, Axel A. ; Goldman, Maria Helena S ; Kim, Heenam ; Nierman, William C. ; Goldman, Gustavo H. / Transcriptome analysis of Aspergillus fumigatus exposed to voriconazole. In: Current Genetics. 2006 ; Vol. 50, No. 1. pp. 32-44.
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