Transcriptome landscape of Synechococcus elongatus PCC 7942 for nitrogen starvation responses using RNA-seq

Sun Young Choi, Byeonghyeok Park, In-Geol Choi, Sang Jun Sim, Sun Mi Lee, Youngsoon Um, Han Min Woo

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The development of high-throughput technology using RNA-seq has allowed understanding of cellular mechanisms and regulations of bacterial transcription. In addition, transcriptome analysis with RNA-seq has been used to accelerate strain improvement through systems metabolic engineering. Synechococcus elongatus PCC 7942, a photosynthetic bacterium, has remarkable potential for biochemical and biofuel production due to photoautotrophic cell growth and direct CO2 conversion. Here, we performed a transcriptome analysis of S. elongatus PCC 7942 using RNA-seq to understand the changes of cellular metabolism and regulation for nitrogen starvation responses. As a result, differentially expressed genes (DEGs) were identified and functionally categorized. With mapping onto metabolic pathways, we probed transcriptional perturbation and regulation of carbon and nitrogen metabolisms relating to nitrogen starvation responses. Experimental evidence such as chlorophyll a and phycobilisome content and the measurement of CO2 uptake rate validated the transcriptome analysis. The analysis suggests that S. elongatus PCC 7942 reacts to nitrogen starvation by not only rearranging the cellular transport capacity involved in carbon and nitrogen assimilation pathways but also by reducing protein synthesis and photosynthesis activities.

Original languageEnglish
Article number30584
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 2016 Aug 4

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Synechococcus
Starvation
Transcriptome
Nitrogen
RNA
Gene Expression Profiling
Carbon
Phycobilisomes
Metabolic Engineering
Biofuels
Photosynthesis
Metabolic Networks and Pathways
Technology
Bacteria
Growth
Genes
Proteins

ASJC Scopus subject areas

  • General

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Transcriptome landscape of Synechococcus elongatus PCC 7942 for nitrogen starvation responses using RNA-seq. / Choi, Sun Young; Park, Byeonghyeok; Choi, In-Geol; Sim, Sang Jun; Lee, Sun Mi; Um, Youngsoon; Woo, Han Min.

In: Scientific Reports, Vol. 6, 30584, 04.08.2016.

Research output: Contribution to journalArticle

Choi, Sun Young ; Park, Byeonghyeok ; Choi, In-Geol ; Sim, Sang Jun ; Lee, Sun Mi ; Um, Youngsoon ; Woo, Han Min. / Transcriptome landscape of Synechococcus elongatus PCC 7942 for nitrogen starvation responses using RNA-seq. In: Scientific Reports. 2016 ; Vol. 6.
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