Transcriptomic analysis of Corynebacterium glutamicum in the response to the toxicity of furfural present in lignocellulosic hydrolysates

Hong Sil Park, Youngsoon Um, Sang Jun Sim, Sang Yup Lee, Han Min Woo

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Pretreatment of lignocellulosic biomass by acid hydrolysis generates growth inhibitors such as furfural, 5-hydroxymethylfurfural, and acetic acid. Among the inhibitors, furfural strongly inhibits cell growth, our objective was to identify the furfural stimulon of an amino acid producer Corynebacterium glutamicum using transcriptomic analysis. As a result, 182 up-regulated (2-fold) genes and 81 down-regulated (0.5-fold) genes were identified for all furfural stress conditions (6.5 mM, 13 mM, and 20 mM). Based on the functional categories of CoryneRegNet database, the furfural stimulon was related to genetic responses of oxidative stress, SOS responses, and anaerobic respiratory metabolism. To overcome the furfural toxicity, furfural-responsive efflux-like permease encoded by the cg1661 gene was overexpressed, resulting that the optical density at 600 nm was 1.54-fold enhanced over the control at 12 h. This study provides alternative strategy of metabolic engineering for biological detoxification in addition to overexpression of NADH-dependent reductases.

Original languageEnglish
Pages (from-to)347-356
Number of pages10
JournalProcess Biochemistry
Volume50
Issue number3
DOIs
Publication statusPublished - 2015

Fingerprint

Corynebacterium glutamicum
Furaldehyde
Furfural
Toxicity
Genes
Anaerobiosis
Metabolic engineering
Metabolic Engineering
Growth Inhibitors
Detoxification
Oxidative stress
Density (optical)
Membrane Transport Proteins
Cell growth
Metabolism
Acetic acid
Acetic Acid
NAD
Biomass
Amino acids

Keywords

  • Corynebacterium glutamicum
  • DNA microarray
  • Efflux-like permease
  • Furfural
  • Metabolic engineering

ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Transcriptomic analysis of Corynebacterium glutamicum in the response to the toxicity of furfural present in lignocellulosic hydrolysates. / Park, Hong Sil; Um, Youngsoon; Sim, Sang Jun; Lee, Sang Yup; Woo, Han Min.

In: Process Biochemistry, Vol. 50, No. 3, 2015, p. 347-356.

Research output: Contribution to journalArticle

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