Transcriptomic study for screening genes involved in the oxidative bioconversions of Streptomyces avermitilis

Hyo Jeong Kim, Kwon Young Choi, Da Hye Jung, Joon Young Jung, Eun Ok Jung, Yung Hun Yang, Byung Gee Kim, Min-Kyu Oh

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Streptomyces avermitilis is a well known organism producing avermectin antibiotics, and has been utilized as an industrial host for oxidation bioconversion processes. Recently, gene screening strategies related to bioconversions have received much focus, as attempts are made to optimize oxidation and biodegradation pathways to maximize yield and productivity. Here, we have demonstrated the oxidative metabolisms of three molecules, daidzein, p-coumaric acid and mevastatin, where S. aver-mitilis converted each substrate to 3′,4′,7-trihydroxyisof-lavone, caffeic acid and hydroxyl-mevastatin to yield 9.3, 32.5 and 15.0 %, respectively. Microarray technology was exploited to investigate genome-wide analysis of gene expression changes, which were induced upon the addition of each substrate. Cytochrome P450 hydroxylases (pteC, cyp28 and olmB), diooxygenases (xylE, cdo1 and putatives) and LuxAB-like oxygenase were identified. One of them, cyp28, was indeed a gene encoding P450 hydroxylase responsible for the oxidative reaction of daidzein. Furthermore, possible electron transfer chain (fdrC → pteE → pteC) supporting cytochrome P450 dependent hydroxylation of daidzein has been suggested based on the interpretation of expression profiles. The result provided a potential application of transcriptomic study on uncovering enzymes involved in oxidative bioconversions of S. avermitilis.

Original languageEnglish
Pages (from-to)1621-1630
Number of pages10
JournalBioprocess and Biosystems Engineering
Volume36
Issue number11
DOIs
Publication statusPublished - 2013 Nov 1

Fingerprint

Bioconversion
Streptomyces
Screening
N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase
Genes
Mixed Function Oxygenases
Cytochrome P-450 Enzyme System
Oxidation
Oxygenases
Hydroxylation
Gene encoding
Acids
Antibiotics
Substrates
Microarrays
Biodegradation
Metabolism
Gene expression
Hydroxyl Radical
Enzymes

Keywords

  • Biotransformation
  • Daidzein
  • Streptomyces avermitilis
  • Transcriptomics

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Transcriptomic study for screening genes involved in the oxidative bioconversions of Streptomyces avermitilis. / Kim, Hyo Jeong; Choi, Kwon Young; Jung, Da Hye; Jung, Joon Young; Jung, Eun Ok; Yang, Yung Hun; Kim, Byung Gee; Oh, Min-Kyu.

In: Bioprocess and Biosystems Engineering, Vol. 36, No. 11, 01.11.2013, p. 1621-1630.

Research output: Contribution to journalArticle

Kim, Hyo Jeong ; Choi, Kwon Young ; Jung, Da Hye ; Jung, Joon Young ; Jung, Eun Ok ; Yang, Yung Hun ; Kim, Byung Gee ; Oh, Min-Kyu. / Transcriptomic study for screening genes involved in the oxidative bioconversions of Streptomyces avermitilis. In: Bioprocess and Biosystems Engineering. 2013 ; Vol. 36, No. 11. pp. 1621-1630.
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