Transesterification of conjugated linoleic acid and tricaprylin by lipases in organic solvents

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Lipase-catalyzed transesterification of tricaprylin with conjugated linoleic acid (CLA) ethyl ester was performed to produce triacylglycerols containing conjugated linoleic acid. Six commercially available lipases, and seven solvents were screened for their ability to incorporate CLA into tricaprylin. The transesterification reaction was performed by incubating a 1:2 mole ratio of tricaprylin and CLA ethyl ester in 3 ml solvents at 55°C, and the products were analyzed by gas chromatography. Three lipases, Novozym 435, Lipozym IM and lipase PS-C were chosen to allow a comparison of transesterification activity in our model reaction. The highest CLA incorporation with Novozym 435 and Lipozym IM was achieved in hexane while isooctane produced the highest CLA incorporation with lipase PS-C. Lipase PS-C gave higher CLA incorporation into tricaprylin when fatty acid was used as the acyl donor than other lipases did. Lipozym IM and lipase PS-C had not restrict specificity to sn-1, 3 positions, even though they had high specificity at sn-1, 3 positions. Novozym 435 among lipases tested was the most effective on the incorporation of CLA into tricaprylin.

Original languageEnglish
Pages (from-to)301-306
Number of pages6
JournalFood Research International
Volume34
Issue number4
DOIs
Publication statusPublished - 2001 May 15

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Conjugated Linoleic Acids
transesterification
conjugated linoleic acid
Lipase
esters
Esters
tricaprylin
Hexanes
hexane
Gas Chromatography
gas chromatography
triacylglycerols
Triglycerides
Fatty Acids
fatty acids

Keywords

  • Acyl donor
  • CLA
  • Hexane
  • Lipase
  • Transesterification

ASJC Scopus subject areas

  • Food Science

Cite this

Transesterification of conjugated linoleic acid and tricaprylin by lipases in organic solvents. / Kim, In-Hwan; Yoon, C. S.; Lee, Kwang Won.

In: Food Research International, Vol. 34, No. 4, 15.05.2001, p. 301-306.

Research output: Contribution to journalArticle

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