Transesterification of waste cooking oil by an organic solvent-tolerant alkaline lipase from Streptomyces sp. CS273

Poonam Mander, Hah Young Yoo, Seung Wook Kim, Yun Hee Choi, Seung Sik Cho, Jin Cheol Yoo

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The aim of this present study was to produce a microbial enzyme that can potentially be utilized for the enzymatic transesterification of waste cooking oil. To that end, an extracellular lipase was isolated and purified from the culture broth of Streptomyces sp. CS273. The molecular mass of purified lipase was estimated to be 36.55 kDa by SDS PAGE. The optimum lipolytic activity was obtained at alkaline pH 8.0 to 8.5 and temperature 40°C, while the enzyme was stable in the pH range 7.0∼9.0 and at temperature ≤40°C. The lipase showed highest hydrolytic activity towards p-nitrophenyl myristate (C14). The lipase activity was enhanced by several salts and detergents including NaCl, MnSo4, and deoxy cholic acid, while phenylmethylsulfonyl fluoride at concentration 10 mM inhibited the activity. The lipase showed tolerance towards different organic solvents including ethanol and methanol which are commonly used in transesterification reactions to displace alcohol from triglycerides (ester) contained in renewable resources to yield fatty acid alkyl esters known as biodiesel. Applicability of the lipase in transesterification of waste cooking oil was confirmed by gas chromatography mass spectrometry analysis.

Original languageEnglish
Pages (from-to)1377-1389
Number of pages13
JournalApplied Biochemistry and Biotechnology
Volume172
Issue number3
DOIs
Publication statusPublished - 2014 Jan 1

Fingerprint

Transesterification
Cooking
Lipases
Streptomyces
Lipase
Organic solvents
Oils
Esters
Enzymes
Phenylmethylsulfonyl Fluoride
Cholic Acid
Temperature
Biofuels
Detergents
Myristic Acid
Molecular mass
Biodiesel
Fatty acids
Gas chromatography
Gas Chromatography-Mass Spectrometry

Keywords

  • Alkaline lipase
  • Biodiesel
  • Streptomyces
  • Transesterification
  • Waste cooking oil

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Biology

Cite this

Transesterification of waste cooking oil by an organic solvent-tolerant alkaline lipase from Streptomyces sp. CS273. / Mander, Poonam; Yoo, Hah Young; Kim, Seung Wook; Choi, Yun Hee; Cho, Seung Sik; Yoo, Jin Cheol.

In: Applied Biochemistry and Biotechnology, Vol. 172, No. 3, 01.01.2014, p. 1377-1389.

Research output: Contribution to journalArticle

Mander, Poonam ; Yoo, Hah Young ; Kim, Seung Wook ; Choi, Yun Hee ; Cho, Seung Sik ; Yoo, Jin Cheol. / Transesterification of waste cooking oil by an organic solvent-tolerant alkaline lipase from Streptomyces sp. CS273. In: Applied Biochemistry and Biotechnology. 2014 ; Vol. 172, No. 3. pp. 1377-1389.
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