Transfer of Tetracycline Resistance Genes with Aggregation Substance in Food-Borne Enterococcus faecalis

Jong Mi Choi, Gun-Jo Woo

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Enterococcus faecalis has the ability to conjugate with the aid of aggregation substance (AS) and inducible sex pheromones to exchange genetic elements in food matrix. To evaluate the food safety condition and the transferable factor, 250 tetracycline-resistant food-borne E. faecalis were collected in Korea. Among the isolates, a majority of tetracycline-resistant isolates (49.6 %) harbored both the tet(M) and tet(L) genes together, followed by tet(M) (19.6 %), and tet(L) (6.8 %) alone. Also, we found the combination of tet(L)/tet(M)/tet(O) or tet(M)/tet(O). We identified two tet(S) genes including the isolate carrying tet(M) + tet(S) genes. Additionally, most E. faecalis were positive for cpd and ccf (both 96.8 %) followed by cob (57.2 %). Through mating experiments, we confirmed E. faecalis possessing the Int-Tn gene and/or any AS gene successfully transferred tet genes to JH2-2 E. faecalis, whereas neither E. faecalis carrying AS genes nor the Int-Tn gene showed the conjugation. Pulsed-field gel electrophoresis results supported a distinct pattern, implying transfer of genetic information. Our study revealed a high occurrence of tetracycline resistance genes in E. faecalis from various foods. The widespread dissemination of tetracycline resistance genes would be promoted to transfer tetracycline resistance genes by pheromone-mediated conjugation systems.

Original languageEnglish
Pages (from-to)476-484
Number of pages9
JournalCurrent Microbiology
Volume70
Issue number4
DOIs
Publication statusPublished - 2015

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Tetracycline Resistance
Enterococcus faecalis
Food
Genes
Tetracycline
Sex Attractants
Food Safety
Pulsed Field Gel Electrophoresis
Pheromones
Korea

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Transfer of Tetracycline Resistance Genes with Aggregation Substance in Food-Borne Enterococcus faecalis. / Choi, Jong Mi; Woo, Gun-Jo.

In: Current Microbiology, Vol. 70, No. 4, 2015, p. 476-484.

Research output: Contribution to journalArticle

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