Transforming growth factor-β-induced protein (TGFBIp/β ig-h3) activates platelets and promotes thrombogenesis

Ha Jeong Kim, Pan Kyung Kim, Sang Mun Bae, Hye Nam Son, Debraj Singh Thoudam, Jung Eun Kim, Byung Heon Lee, Rang Woon Park, In-San Kim

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Transforming growth factor-β-induced protein (TGFBIp)/βig-h3 is a 68-kDa extracellular matrix protein that is functionally associated with the adhesion, migration, proliferation, and differentiation of various cells. The presence of TGFBIp in platelets led us to study the role of this protein in the regulation of platelet functions. Upon activation, platelet TGFBIp was released and associated with the platelets. TGFBIp mediates not only the adhesion and spread of platelets but also activates them, resulting in phosphatidylserine exposure, α-granule secretion, and increased integrin affinity. The fasciclin 1 domains of TGFBIp are mainly responsible for the activation of platelets. TGFBIp promotes thrombus formation on type I fibrillar collagen under flow conditions in vitro and induces pulmonary embolism in mice. Moreover, transgenic mice, which have approximately a 1.7-fold greater blood TGFBIp concentration, are significantly more susceptible to collagen- and epinephrine-induced pulmonary embolism than wild-type mice. These results suggest that TGFBIp, a human platelet protein, plays important roles in platelet activation and thrombus formation. Our findings will increase our understanding of the novel mechanism of platelet activation, contributing to a better understanding of thrombotic pathways and the development of new antithrombotic therapies.

Original languageEnglish
Pages (from-to)5206-5215
Number of pages10
JournalBlood
Volume114
Issue number25
DOIs
Publication statusPublished - 2009 Dec 10
Externally publishedYes

Fingerprint

Transforming Growth Factors
Platelets
Blood Platelets
Platelet Activation
Chemical activation
Pulmonary Embolism
Thrombosis
Fibrillar Collagens
Extracellular Matrix Proteins
Phosphatidylserines
Adhesion
Integrins
Transgenic Mice
Epinephrine
betaIG-H3 protein
Cell Differentiation
Proteins
Collagen
Blood
Cells

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Transforming growth factor-β-induced protein (TGFBIp/β ig-h3) activates platelets and promotes thrombogenesis. / Kim, Ha Jeong; Kim, Pan Kyung; Bae, Sang Mun; Son, Hye Nam; Thoudam, Debraj Singh; Kim, Jung Eun; Lee, Byung Heon; Park, Rang Woon; Kim, In-San.

In: Blood, Vol. 114, No. 25, 10.12.2009, p. 5206-5215.

Research output: Contribution to journalArticle

Kim, HJ, Kim, PK, Bae, SM, Son, HN, Thoudam, DS, Kim, JE, Lee, BH, Park, RW & Kim, I-S 2009, 'Transforming growth factor-β-induced protein (TGFBIp/β ig-h3) activates platelets and promotes thrombogenesis', Blood, vol. 114, no. 25, pp. 5206-5215. https://doi.org/10.1182/blood-2009-03-212415
Kim, Ha Jeong ; Kim, Pan Kyung ; Bae, Sang Mun ; Son, Hye Nam ; Thoudam, Debraj Singh ; Kim, Jung Eun ; Lee, Byung Heon ; Park, Rang Woon ; Kim, In-San. / Transforming growth factor-β-induced protein (TGFBIp/β ig-h3) activates platelets and promotes thrombogenesis. In: Blood. 2009 ; Vol. 114, No. 25. pp. 5206-5215.
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