Transgelin Depletion is Critical for the TGFβ1-mediated Initiation of PLCγ1-Cofilin-driven Morphological and Migratory Changes in MDA-MB-231 Cells

Bernardo R. Raymundo, In Rok Oh, Mi Jung Kim, Chan Wha Kim

Research output: Contribution to journalArticle

Abstract

The role of transgelin (TAGLN) in cancer has been discussed; however, the mechanisms underlying its regulation and correlation with MDA-MB-231 cell plasticity and migratory patterns remain unclear. We generated stable TAGLN-knockdown MDA-MB-231 cells and treated them with phorbol 12-myristate 13-acetate or transforming growth factor (TGF)-β. Chemotaxis, morphology, and invasion were assayed using three-dimensional matrices to evaluate cytoskeletal remodeling and migratory changes. Wound healing assays were conducted using cell inserts. TAGLN knockdown cells exhibited altered morphology due to cytoskeletal remodeling, yet only untreated and TGFβ1-treated cells exhibited enhanced migration. Untreated and TGFβ1-treated TAGLN knockdown cells showed increased N-WASP, ROCK1, and ROCK2 protein levels, which induce cytoskeletal remodeling. Evaluating phospholipase Cγ1 (PLCγ1)-cofilin signaling-related proteins revealed that only TGFβ1-treated TAGLN knockdown cells were influenced by PLCγ1-cofilin signaling. Taken together, TAGLN knockdown is necessary for the TGFβ1-mediated activation of PLCγ1-cofilin pathway-driven amoeboid morphology and enhanced migratory properties in MDA-MB-231 cells.

Original languageEnglish
Pages (from-to)1191-1201
Number of pages11
JournalBulletin of the Korean Chemical Society
Volume40
Issue number12
DOIs
Publication statusPublished - 2019 Dec 1

Fingerprint

Actin Depolymerizing Factors
Transforming Growth Factors
Plasticity
transgelin
phospholipase C1
Assays
Proteins
Acetates
Chemical activation
Cells

Keywords

  • Actin cytoskeleton
  • Cofilin
  • Plasticity

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Transgelin Depletion is Critical for the TGFβ1-mediated Initiation of PLCγ1-Cofilin-driven Morphological and Migratory Changes in MDA-MB-231 Cells. / Raymundo, Bernardo R.; Oh, In Rok; Kim, Mi Jung; Kim, Chan Wha.

In: Bulletin of the Korean Chemical Society, Vol. 40, No. 12, 01.12.2019, p. 1191-1201.

Research output: Contribution to journalArticle

@article{41129170a7774417a38006a0aa3d88be,
title = "Transgelin Depletion is Critical for the TGFβ1-mediated Initiation of PLCγ1-Cofilin-driven Morphological and Migratory Changes in MDA-MB-231 Cells",
abstract = "The role of transgelin (TAGLN) in cancer has been discussed; however, the mechanisms underlying its regulation and correlation with MDA-MB-231 cell plasticity and migratory patterns remain unclear. We generated stable TAGLN-knockdown MDA-MB-231 cells and treated them with phorbol 12-myristate 13-acetate or transforming growth factor (TGF)-β. Chemotaxis, morphology, and invasion were assayed using three-dimensional matrices to evaluate cytoskeletal remodeling and migratory changes. Wound healing assays were conducted using cell inserts. TAGLN knockdown cells exhibited altered morphology due to cytoskeletal remodeling, yet only untreated and TGFβ1-treated cells exhibited enhanced migration. Untreated and TGFβ1-treated TAGLN knockdown cells showed increased N-WASP, ROCK1, and ROCK2 protein levels, which induce cytoskeletal remodeling. Evaluating phospholipase Cγ1 (PLCγ1)-cofilin signaling-related proteins revealed that only TGFβ1-treated TAGLN knockdown cells were influenced by PLCγ1-cofilin signaling. Taken together, TAGLN knockdown is necessary for the TGFβ1-mediated activation of PLCγ1-cofilin pathway-driven amoeboid morphology and enhanced migratory properties in MDA-MB-231 cells.",
keywords = "Actin cytoskeleton, Cofilin, Plasticity",
author = "Raymundo, {Bernardo R.} and Oh, {In Rok} and Kim, {Mi Jung} and Kim, {Chan Wha}",
year = "2019",
month = "12",
day = "1",
doi = "10.1002/bkcs.11900",
language = "English",
volume = "40",
pages = "1191--1201",
journal = "Bulletin of the Korean Chemical Society",
issn = "0253-2964",
publisher = "Wiley-Blackwell",
number = "12",

}

TY - JOUR

T1 - Transgelin Depletion is Critical for the TGFβ1-mediated Initiation of PLCγ1-Cofilin-driven Morphological and Migratory Changes in MDA-MB-231 Cells

AU - Raymundo, Bernardo R.

AU - Oh, In Rok

AU - Kim, Mi Jung

AU - Kim, Chan Wha

PY - 2019/12/1

Y1 - 2019/12/1

N2 - The role of transgelin (TAGLN) in cancer has been discussed; however, the mechanisms underlying its regulation and correlation with MDA-MB-231 cell plasticity and migratory patterns remain unclear. We generated stable TAGLN-knockdown MDA-MB-231 cells and treated them with phorbol 12-myristate 13-acetate or transforming growth factor (TGF)-β. Chemotaxis, morphology, and invasion were assayed using three-dimensional matrices to evaluate cytoskeletal remodeling and migratory changes. Wound healing assays were conducted using cell inserts. TAGLN knockdown cells exhibited altered morphology due to cytoskeletal remodeling, yet only untreated and TGFβ1-treated cells exhibited enhanced migration. Untreated and TGFβ1-treated TAGLN knockdown cells showed increased N-WASP, ROCK1, and ROCK2 protein levels, which induce cytoskeletal remodeling. Evaluating phospholipase Cγ1 (PLCγ1)-cofilin signaling-related proteins revealed that only TGFβ1-treated TAGLN knockdown cells were influenced by PLCγ1-cofilin signaling. Taken together, TAGLN knockdown is necessary for the TGFβ1-mediated activation of PLCγ1-cofilin pathway-driven amoeboid morphology and enhanced migratory properties in MDA-MB-231 cells.

AB - The role of transgelin (TAGLN) in cancer has been discussed; however, the mechanisms underlying its regulation and correlation with MDA-MB-231 cell plasticity and migratory patterns remain unclear. We generated stable TAGLN-knockdown MDA-MB-231 cells and treated them with phorbol 12-myristate 13-acetate or transforming growth factor (TGF)-β. Chemotaxis, morphology, and invasion were assayed using three-dimensional matrices to evaluate cytoskeletal remodeling and migratory changes. Wound healing assays were conducted using cell inserts. TAGLN knockdown cells exhibited altered morphology due to cytoskeletal remodeling, yet only untreated and TGFβ1-treated cells exhibited enhanced migration. Untreated and TGFβ1-treated TAGLN knockdown cells showed increased N-WASP, ROCK1, and ROCK2 protein levels, which induce cytoskeletal remodeling. Evaluating phospholipase Cγ1 (PLCγ1)-cofilin signaling-related proteins revealed that only TGFβ1-treated TAGLN knockdown cells were influenced by PLCγ1-cofilin signaling. Taken together, TAGLN knockdown is necessary for the TGFβ1-mediated activation of PLCγ1-cofilin pathway-driven amoeboid morphology and enhanced migratory properties in MDA-MB-231 cells.

KW - Actin cytoskeleton

KW - Cofilin

KW - Plasticity

UR - http://www.scopus.com/inward/record.url?scp=85074870703&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85074870703&partnerID=8YFLogxK

U2 - 10.1002/bkcs.11900

DO - 10.1002/bkcs.11900

M3 - Article

AN - SCOPUS:85074870703

VL - 40

SP - 1191

EP - 1201

JO - Bulletin of the Korean Chemical Society

JF - Bulletin of the Korean Chemical Society

SN - 0253-2964

IS - 12

ER -