Transgelin promotes migration and invasion of cancer stem cells

Eun Kyung Lee, Gi Yeon Han, Hye Won Park, Yeo Ju Song, Chan Wha Kim

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Recent studies have suggested the existence of a small subset of cancer cells called cancer stem cells (CSCs), which possess the ability to initiate malignancies, promote tumor formation, drive metastasis, and evade conventional chemotherapies. Elucidation of the specific signaling pathway and mechanism underlying the action of CSCs might improve the efficacy of cancer treatments. In this study, we analyzed differentially expressed proteins between tumerigenic and nontumorigenic cells isolated from the human hepatocellular carcinoma (HCC) cell line, Huh7, via proteomic analysis to identify proteins correlated with specific features of CSCs. The expression level of Transgelin was 25-fold higher in tumorigenic cells than nontumorigenic cells. Similar results were also observed in tumorigenic cells derived from colorectal adenocarcinoma and prostate carcinoma. More importantly, the elevated levels of Transgelin significantly increased the invasiveness of tumorigenic cells, whereas reduced levels decreased the invasive potential. Moreover, in tumors derived from Huh7-induced xenografts, Transgelin was also co-expressed with CXCR4, which is responsible for tumor invasion. Taken together, these results indicate that the metastatic potential of CSCs arises from highly expressed Transgelin.

Original languageEnglish
Pages (from-to)5108-5117
Number of pages10
JournalJournal of Proteome Research
Volume9
Issue number10
DOIs
Publication statusPublished - 2010 Oct 1

Keywords

  • 2-dimensional electrophoresis
  • cancer stem cell
  • invasion
  • migration
  • transgelin

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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  • Cite this

    Lee, E. K., Han, G. Y., Park, H. W., Song, Y. J., & Kim, C. W. (2010). Transgelin promotes migration and invasion of cancer stem cells. Journal of Proteome Research, 9(10), 5108-5117. https://doi.org/10.1021/pr100378z