TY - JOUR
T1 - Transgenerational effects of polyethylene microplastic fragments containing benzophenone-3 additive in Daphnia magna
AU - Song, Jinyoung
AU - Kim, Changhae
AU - Na, Joorim
AU - Sivri, Nüket
AU - Samanta, Palas
AU - Jung, Jinho
N1 - Funding Information:
This study was supported by a grant from the National Research Foundation of Korea (NRF), funded by the Korean government ( NRF-2019R1A2C1002890 ).
Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022/8/15
Y1 - 2022/8/15
N2 - Maternal exposure to microplastics (MPs) plays an important role in the fitness of unexposed progeny. In this study, the transgenerational effects of polyethylene MP fragments (17.35 ± 5.50 µm) containing benzophenone-3 (BP-3; 2.85 ± 0.16% w/w) on chronic toxicity (21 d) in Daphnia magna were investigated across four generations. Only D. magna in the F0 generation was exposed to MP fragments, MP/BP-3 fragments, and BP-3 leachate to identify the transgenerational effect in the F3 generation. The mortality of D. magna induced by MP and MP/BP-3 fragments was recovered in the F3 generation, but somatic growth and reproduction significantly decreased compared to the control. Additionally, reproduction of D. magna exposed to BP-3 leachate significantly decreased in the F3 generation. These findings confirmed the transgenerational effects of MP fragment and BP-3 additive on D. magna. Particularly, the adverse effect on D. magna reproduction seemed to be cumulative across four generations for MP/BP-3 fragments, while it was an acclimation trend for BP-3 leachate. However, there was no significant difference in global DNA methylation in D. magna across four generations, thus requiring a gene-specific DNA methylation study to identify different epigenetic transgenerational inheritance.
AB - Maternal exposure to microplastics (MPs) plays an important role in the fitness of unexposed progeny. In this study, the transgenerational effects of polyethylene MP fragments (17.35 ± 5.50 µm) containing benzophenone-3 (BP-3; 2.85 ± 0.16% w/w) on chronic toxicity (21 d) in Daphnia magna were investigated across four generations. Only D. magna in the F0 generation was exposed to MP fragments, MP/BP-3 fragments, and BP-3 leachate to identify the transgenerational effect in the F3 generation. The mortality of D. magna induced by MP and MP/BP-3 fragments was recovered in the F3 generation, but somatic growth and reproduction significantly decreased compared to the control. Additionally, reproduction of D. magna exposed to BP-3 leachate significantly decreased in the F3 generation. These findings confirmed the transgenerational effects of MP fragment and BP-3 additive on D. magna. Particularly, the adverse effect on D. magna reproduction seemed to be cumulative across four generations for MP/BP-3 fragments, while it was an acclimation trend for BP-3 leachate. However, there was no significant difference in global DNA methylation in D. magna across four generations, thus requiring a gene-specific DNA methylation study to identify different epigenetic transgenerational inheritance.
KW - Chronic toxicity
KW - Microplastic
KW - Multigeneration
KW - Plastic additive
KW - Zooplankton
UR - http://www.scopus.com/inward/record.url?scp=85131429015&partnerID=8YFLogxK
U2 - 10.1016/j.jhazmat.2022.129225
DO - 10.1016/j.jhazmat.2022.129225
M3 - Article
C2 - 35739745
AN - SCOPUS:85131429015
SN - 0304-3894
VL - 436
JO - Journal of Hazardous Materials
JF - Journal of Hazardous Materials
M1 - 129225
ER -