Transgenic mice expressing yellow fluorescent protein under control of the human tyrosine hydroxylase promoter

Eun Yang Choi, Jae Won Yang, Myung Sun Park, Woong Sun, Hyun Kim, Seung U. Kim, Myung Ae Lee

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Pathogenesis of Parkinson's disease and related catecholaminergic neurological disorders is closely associated with changes in the levels of tyrosine hydroxylase (TH). Therefore, investigation of the regulation of the TH gene system should assist in understanding the pathomechanisms involved in these neurological disorders. To identify regulatory domains that direct human TH expression in the central nervous system (CNS), we generated two transgenic mouse lines in which enhanced yellow fluorescent protein (EYFP) is expressed under the control of either 3.2-kb (hTHP-EYFP construct) human TH promoter or 3.2-kb promoter with 2-kb 3′-flanking regions (hTHP-ex3-EYFP construct) of the TH gene. In the adult transgenic mouse brain, the hTHP-EYFP construct directs neuron-specific EYFP expression in various CNS areas, such as olfactory bulb, striatum, interpeduncular nucleus, cerebral cortex, hippocampus, and particularly dentate gyrus. Although these EYFP-positive cells were identified as mature neurons, few EYFP-positive cells were TH-positive neurons. On the other hand, we could detect the EYFP mRNA expression in a subset of neurons in the olfactory bulb, midbrain, and cerebellum, in which expression of endogenous TH is enriched, with hTHP-ex3-EYFP transgenic mice. These results indicate that the 3.2-kb sequence upstream of the TH gene is not sufficient for proper expression and that the 2-kb sequence from the translation start site to exon 3 is necessary for expression of EYFP in a subset of catecholaminergic neurons.

Original languageEnglish
Pages (from-to)1949-1959
Number of pages11
JournalJournal of Neuroscience Research
Volume90
Issue number10
DOIs
Publication statusPublished - 2012 Oct 1

Fingerprint

Tyrosine 3-Monooxygenase
Transgenic Mice
Proteins
Neurons
Olfactory Bulb
Nervous System Diseases
Central Nervous System
3' Flanking Region
Genes
Dentate Gyrus
Mesencephalon
Cerebral Cortex
Cerebellum
Parkinson Disease
Exons
Hippocampus
Messenger RNA
Brain

Keywords

  • Catecholaminergic neuron
  • EYFP
  • Promoter
  • Transgenic mice
  • Tyrosine hydroxylase

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Transgenic mice expressing yellow fluorescent protein under control of the human tyrosine hydroxylase promoter. / Choi, Eun Yang; Yang, Jae Won; Park, Myung Sun; Sun, Woong; Kim, Hyun; Kim, Seung U.; Lee, Myung Ae.

In: Journal of Neuroscience Research, Vol. 90, No. 10, 01.10.2012, p. 1949-1959.

Research output: Contribution to journalArticle

Choi, Eun Yang ; Yang, Jae Won ; Park, Myung Sun ; Sun, Woong ; Kim, Hyun ; Kim, Seung U. ; Lee, Myung Ae. / Transgenic mice expressing yellow fluorescent protein under control of the human tyrosine hydroxylase promoter. In: Journal of Neuroscience Research. 2012 ; Vol. 90, No. 10. pp. 1949-1959.
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