Transglucosylation of caffeic acid by a recombinant sucrose phosphorylase in aqueous buffer and aqueous-supercritical CO2 media

Min Hye Shin; Nam Yong Cheong; Jong Hoon Lee; Kyoung Heon Kim

doi:10.1016/j.foodchem.2009.01.013

Transglucosylation of caffeic acid by a recombinant sucrose phosphorylase in aqueous buffer and aqueous-supercritical CO₂ media

Min Hye Shin, Nam Yong Cheong, Jong Hoon Lee, Kyoung Heon Kim

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Polyphenolic compounds, such as caffeic acid, offer many health benefits, but their industrial applications are limited because of their low solubility in water and instability under UV light or heat. In this study, we enzymatically produced caffeic acid glucosides, in both aqueous buffer and aqueous-supercritical carbon dioxide (SC-CO₂) media, using a recombinant sucrose phosphorylase (SPase) from Bifidobacterium longum. By using LC/MS/MS analysis, we verified that the enzymatic reaction products were caffeic acid monoglucosides and diglucosides. Under SC-CO₂, the amounts of the reaction products from the enzyme reaction were smaller than those in the aqueous reaction medium. However, this is the first report of the transglucosylation of caffeic acid by SPase, and also the first enzymatic reaction with phenolic compounds conducted in a SC-CO₂ phase.

Original language	English
Pages (from-to)	1028-1033
Number of pages	6
Journal	Food Chemistry
Volume	115
Issue number	3
DOIs	https://doi.org/10.1016/j.foodchem.2009.01.013
Publication status	Published - 2009 Aug 1

Keywords

Bifidobacterium longum
Caffeic acid
Caffeic acid glucoside
LC/MS/MS
Sucrose phosphorylase
Supercritical carbon dioxide
Transglucosylation

ASJC Scopus subject areas

Analytical Chemistry
Food Science

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title = "Transglucosylation of caffeic acid by a recombinant sucrose phosphorylase in aqueous buffer and aqueous-supercritical CO2 media",

abstract = "Polyphenolic compounds, such as caffeic acid, offer many health benefits, but their industrial applications are limited because of their low solubility in water and instability under UV light or heat. In this study, we enzymatically produced caffeic acid glucosides, in both aqueous buffer and aqueous-supercritical carbon dioxide (SC-CO2) media, using a recombinant sucrose phosphorylase (SPase) from Bifidobacterium longum. By using LC/MS/MS analysis, we verified that the enzymatic reaction products were caffeic acid monoglucosides and diglucosides. Under SC-CO2, the amounts of the reaction products from the enzyme reaction were smaller than those in the aqueous reaction medium. However, this is the first report of the transglucosylation of caffeic acid by SPase, and also the first enzymatic reaction with phenolic compounds conducted in a SC-CO2 phase.",

keywords = "Bifidobacterium longum, Caffeic acid, Caffeic acid glucoside, LC/MS/MS, Sucrose phosphorylase, Supercritical carbon dioxide, Transglucosylation",

author = "Shin, {Min Hye} and Cheong, {Nam Yong} and Lee, {Jong Hoon} and Kim, {Kyoung Heon}",

note = "Funding Information: This work was supported by a Grant (20080401-034-049) from BioGreen 21 Program, Rural Development Administration, Republic of Korea. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.",

year = "2009",

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doi = "10.1016/j.foodchem.2009.01.013",

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AU - Shin, Min Hye

AU - Cheong, Nam Yong

AU - Lee, Jong Hoon

AU - Kim, Kyoung Heon

N1 - Funding Information: This work was supported by a Grant (20080401-034-049) from BioGreen 21 Program, Rural Development Administration, Republic of Korea. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.

PY - 2009/8/1

Y1 - 2009/8/1

N2 - Polyphenolic compounds, such as caffeic acid, offer many health benefits, but their industrial applications are limited because of their low solubility in water and instability under UV light or heat. In this study, we enzymatically produced caffeic acid glucosides, in both aqueous buffer and aqueous-supercritical carbon dioxide (SC-CO2) media, using a recombinant sucrose phosphorylase (SPase) from Bifidobacterium longum. By using LC/MS/MS analysis, we verified that the enzymatic reaction products were caffeic acid monoglucosides and diglucosides. Under SC-CO2, the amounts of the reaction products from the enzyme reaction were smaller than those in the aqueous reaction medium. However, this is the first report of the transglucosylation of caffeic acid by SPase, and also the first enzymatic reaction with phenolic compounds conducted in a SC-CO2 phase.

AB - Polyphenolic compounds, such as caffeic acid, offer many health benefits, but their industrial applications are limited because of their low solubility in water and instability under UV light or heat. In this study, we enzymatically produced caffeic acid glucosides, in both aqueous buffer and aqueous-supercritical carbon dioxide (SC-CO2) media, using a recombinant sucrose phosphorylase (SPase) from Bifidobacterium longum. By using LC/MS/MS analysis, we verified that the enzymatic reaction products were caffeic acid monoglucosides and diglucosides. Under SC-CO2, the amounts of the reaction products from the enzyme reaction were smaller than those in the aqueous reaction medium. However, this is the first report of the transglucosylation of caffeic acid by SPase, and also the first enzymatic reaction with phenolic compounds conducted in a SC-CO2 phase.

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