Transglucosylation of caffeic acid by a recombinant sucrose phosphorylase in aqueous buffer and aqueous-supercritical CO2 media

Min Hye Shin, Nam Yong Cheong, Jong Hoon Lee, Kyoung Heon Kim

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Polyphenolic compounds, such as caffeic acid, offer many health benefits, but their industrial applications are limited because of their low solubility in water and instability under UV light or heat. In this study, we enzymatically produced caffeic acid glucosides, in both aqueous buffer and aqueous-supercritical carbon dioxide (SC-CO2) media, using a recombinant sucrose phosphorylase (SPase) from Bifidobacterium longum. By using LC/MS/MS analysis, we verified that the enzymatic reaction products were caffeic acid monoglucosides and diglucosides. Under SC-CO2, the amounts of the reaction products from the enzyme reaction were smaller than those in the aqueous reaction medium. However, this is the first report of the transglucosylation of caffeic acid by SPase, and also the first enzymatic reaction with phenolic compounds conducted in a SC-CO2 phase.

Original languageEnglish
Pages (from-to)1028-1033
Number of pages6
JournalFood Chemistry
Volume115
Issue number3
DOIs
Publication statusPublished - 2009 Aug 1

Keywords

  • Bifidobacterium longum
  • Caffeic acid
  • Caffeic acid glucoside
  • LC/MS/MS
  • Sucrose phosphorylase
  • Supercritical carbon dioxide
  • Transglucosylation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

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