TY - JOUR
T1 - Transglucosylation of caffeic acid by a recombinant sucrose phosphorylase in aqueous buffer and aqueous-supercritical CO2 media
AU - Shin, Min Hye
AU - Cheong, Nam Yong
AU - Lee, Jong Hoon
AU - Kim, Kyoung Heon
PY - 2009/8/1
Y1 - 2009/8/1
N2 - Polyphenolic compounds, such as caffeic acid, offer many health benefits, but their industrial applications are limited because of their low solubility in water and instability under UV light or heat. In this study, we enzymatically produced caffeic acid glucosides, in both aqueous buffer and aqueous-supercritical carbon dioxide (SC-CO2) media, using a recombinant sucrose phosphorylase (SPase) from Bifidobacterium longum. By using LC/MS/MS analysis, we verified that the enzymatic reaction products were caffeic acid monoglucosides and diglucosides. Under SC-CO2, the amounts of the reaction products from the enzyme reaction were smaller than those in the aqueous reaction medium. However, this is the first report of the transglucosylation of caffeic acid by SPase, and also the first enzymatic reaction with phenolic compounds conducted in a SC-CO2 phase.
AB - Polyphenolic compounds, such as caffeic acid, offer many health benefits, but their industrial applications are limited because of their low solubility in water and instability under UV light or heat. In this study, we enzymatically produced caffeic acid glucosides, in both aqueous buffer and aqueous-supercritical carbon dioxide (SC-CO2) media, using a recombinant sucrose phosphorylase (SPase) from Bifidobacterium longum. By using LC/MS/MS analysis, we verified that the enzymatic reaction products were caffeic acid monoglucosides and diglucosides. Under SC-CO2, the amounts of the reaction products from the enzyme reaction were smaller than those in the aqueous reaction medium. However, this is the first report of the transglucosylation of caffeic acid by SPase, and also the first enzymatic reaction with phenolic compounds conducted in a SC-CO2 phase.
KW - Bifidobacterium longum
KW - Caffeic acid
KW - Caffeic acid glucoside
KW - LC/MS/MS
KW - Sucrose phosphorylase
KW - Supercritical carbon dioxide
KW - Transglucosylation
UR - http://www.scopus.com/inward/record.url?scp=62249150863&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=62249150863&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2009.01.013
DO - 10.1016/j.foodchem.2009.01.013
M3 - Article
AN - SCOPUS:62249150863
VL - 115
SP - 1028
EP - 1033
JO - Food Chemistry
JF - Food Chemistry
SN - 0308-8146
IS - 3
ER -