Transglycosylation of tagatose with maltotriose by Bacillus stearothermophilus maltogenic amylase (BSMA)

Hoe Jin Roh, Su Cheol Kang, Hee Seob Lee, Doo Kyung Kim, Sung Bae Byun, Sung-Joon Lee, Kwan Hwa Park

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

d-Tagatose was transglycosylated by Bacillus stearothermophilus maltogenic amylase (BSMA), and the physicochemical properties of the transfer products were analyzed. Maltosyl-tagatose was the main product of the transglycosylation reaction using maltotriose as the donor and d-tagatose as the acceptor. Glucosyl-tagatose was produced from maltosyl-tagatose by removal of a glucosyl moiety by glucoamylase. The 13C NMR analysis of glucosyl-tagatose suggested that a linkage was formed between the C-1 carbon of the glucose unit and the C-1 carbon of the tagatose unit, thereby producing 1,1-α-glucosyl- tagatose from the transglycosylation reaction. Hygroscopicity measurements showed that glucosyl-tagatose had greater water sorption than did tagatose or sucrose. The glass transition temperature of glucosyl-tagatose was -29°C, which is considerably higher than that of tagatose, -45°C. Its structure and physicochemical properties suggest that glucosyl-tagatose has potential as a low-calorie sweetener and cryostabilizer.

Original languageEnglish
Pages (from-to)77-82
Number of pages6
JournalTetrahedron Asymmetry
Volume16
Issue number1
DOIs
Publication statusPublished - 2005 Jan 10

Fingerprint

glucan 1,4-alpha-maltohydrolase
stearothermophilus
Geobacillus stearothermophilus
Amylases
Bacillus
Bacilli
hygroscopicity
Carbon
sucrose
carbon
Sugar (sucrose)
products
glucose
linkages
sorption
glass transition temperature
Glucose
Sorption
Nuclear magnetic resonance
nuclear magnetic resonance

ASJC Scopus subject areas

  • Inorganic Chemistry
  • Organic Chemistry
  • Materials Chemistry
  • Drug Discovery

Cite this

Transglycosylation of tagatose with maltotriose by Bacillus stearothermophilus maltogenic amylase (BSMA). / Roh, Hoe Jin; Kang, Su Cheol; Lee, Hee Seob; Kim, Doo Kyung; Byun, Sung Bae; Lee, Sung-Joon; Park, Kwan Hwa.

In: Tetrahedron Asymmetry, Vol. 16, No. 1, 10.01.2005, p. 77-82.

Research output: Contribution to journalArticle

Roh, Hoe Jin ; Kang, Su Cheol ; Lee, Hee Seob ; Kim, Doo Kyung ; Byun, Sung Bae ; Lee, Sung-Joon ; Park, Kwan Hwa. / Transglycosylation of tagatose with maltotriose by Bacillus stearothermophilus maltogenic amylase (BSMA). In: Tetrahedron Asymmetry. 2005 ; Vol. 16, No. 1. pp. 77-82.
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