Abstract
Mitogen-Activated Protein Kinase (MAPK) cascade is one of the main signaling components mediating abiotic and biotic stress and hormone information in plants. Plant MAPK study has been impeded with a genetic approach using a long-term phenotypic analysis in spite of the transient nature of the protein kinase signaling. Arabidopsis leaf mesophyll protoplasts provide a versatile resource for diverse cell-based assays to acquire immediate molecular and biochemical responses with transient expression of MAPK cascade components of interests. Thus, it is an attractive tool for a high-throughput functional analysis of Arabidopsis MAPK cascade signaling. However, transient expression in Arabidopsis mesophyll protoplast (TEAMP) system requires mastered skills for protoplast preparation and handling to achieve steady and stable data. Here, we have described two analytical methods for MAPK cascade signaling using TEAMP system.
| Original language | English |
|---|---|
| Pages (from-to) | 3-12 |
| Number of pages | 10 |
| Journal | Methods in molecular biology (Clifton, N.J.) |
| Volume | 1171 |
| DOIs | |
| Publication status | Published - 2014 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Medicine(all)
Cite this
Transient expression in Arabidopsis leaf mesophyll protoplast system for cell-based functional analysis of MAPK cascades signaling. / Im, Jong H ee; Yoo, Sang-Dong.
In: Methods in molecular biology (Clifton, N.J.), Vol. 1171, 2014, p. 3-12.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transient expression in Arabidopsis leaf mesophyll protoplast system for cell-based functional analysis of MAPK cascades signaling
AU - Im, Jong H ee
AU - Yoo, Sang-Dong
PY - 2014
Y1 - 2014
N2 - Mitogen-Activated Protein Kinase (MAPK) cascade is one of the main signaling components mediating abiotic and biotic stress and hormone information in plants. Plant MAPK study has been impeded with a genetic approach using a long-term phenotypic analysis in spite of the transient nature of the protein kinase signaling. Arabidopsis leaf mesophyll protoplasts provide a versatile resource for diverse cell-based assays to acquire immediate molecular and biochemical responses with transient expression of MAPK cascade components of interests. Thus, it is an attractive tool for a high-throughput functional analysis of Arabidopsis MAPK cascade signaling. However, transient expression in Arabidopsis mesophyll protoplast (TEAMP) system requires mastered skills for protoplast preparation and handling to achieve steady and stable data. Here, we have described two analytical methods for MAPK cascade signaling using TEAMP system.
AB - Mitogen-Activated Protein Kinase (MAPK) cascade is one of the main signaling components mediating abiotic and biotic stress and hormone information in plants. Plant MAPK study has been impeded with a genetic approach using a long-term phenotypic analysis in spite of the transient nature of the protein kinase signaling. Arabidopsis leaf mesophyll protoplasts provide a versatile resource for diverse cell-based assays to acquire immediate molecular and biochemical responses with transient expression of MAPK cascade components of interests. Thus, it is an attractive tool for a high-throughput functional analysis of Arabidopsis MAPK cascade signaling. However, transient expression in Arabidopsis mesophyll protoplast (TEAMP) system requires mastered skills for protoplast preparation and handling to achieve steady and stable data. Here, we have described two analytical methods for MAPK cascade signaling using TEAMP system.
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UR - http://www.scopus.com/inward/citedby.url?scp=84922480023&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-0922-3_1
DO - 10.1007/978-1-4939-0922-3_1
M3 - Article
C2 - 24908115
AN - SCOPUS:84922480023
VL - 1171
SP - 3
EP - 12
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -