Transient receptor potential V2 expressed in sensory neurons is activated by probenecid

Sangsu Bang, Kyung Yoon Kim, Sungjae Yoo, Sang Heon Lee, Sun Wook Hwang

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Temperature-activated transient receptor potential ion channels (thermoTRPs) are known to function as ambient temperature sensors and are also involved in peripheral pain sensation. The thermoTRPs are activated by a variety of chemicals, of which specific activators have been utilized to explore the physiology of particular channels and sensory nerve subtypes. The use of capsaicin for TRPV1 is an exemplary case for nociceptor studies. In contrast, specific agents for another vanilloid subtype channel, TRPV2 have been lacking. Here, we show that probenecid is able to activate TRPV2 using electrophysiological and calcium imaging techniques with TRPV2-expressing HEK293T cells. Five other sensory thermoTRPs-TRPV1, TRPV3, TRPV4, TRPM8 and TRPA1-failed to show a response to this drug in the same heterologous expression system, suggesting that probenecid is a specific activator for TRPV2. Probenecid-evoked responses were also reproduced in a distinct subset of cultured trigeminal neurons that were responsive to 2-aminoethoxydiphenyl borate, a TRPV1-3 activator. The probenecid-sensitive neurons were mainly distributed in a medium to large-diameter population, in agreement with previous observations with TRPV2 immunolocalization. Under inflammation, probenecid elicited nociceptive behaviors in in vivo assays. These results suggest that TRPV2 is specifically activated by probenecid and that this chemical might be useful for investigation of pain-related TRPV2 function.

Original languageEnglish
Pages (from-to)120-125
Number of pages6
JournalNeuroscience Letters
Volume425
Issue number2
DOIs
Publication statusPublished - 2007 Sep 25

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Probenecid
Vasopressin Receptors
Sensory Receptor Cells
Transient Receptor Potential Channels
Neurons
Pain
Nociceptors
Temperature
Capsaicin
Ion Channels
Inflammation
Calcium
Pharmaceutical Preparations
Population

Keywords

  • Pain
  • Probenecid
  • Specific activator
  • Trigeminal neurons
  • TRPV2

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Transient receptor potential V2 expressed in sensory neurons is activated by probenecid. / Bang, Sangsu; Kim, Kyung Yoon; Yoo, Sungjae; Lee, Sang Heon; Hwang, Sun Wook.

In: Neuroscience Letters, Vol. 425, No. 2, 25.09.2007, p. 120-125.

Research output: Contribution to journalArticle

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