Translation initiation on mRNAs bound by nuclear cap-binding protein complex CBP80/20 requires interaction between CBP80/20-dependent translation initiation factor and eukaryotic translation initiation factor 3g

Junho Choe, Nara Oh, Sungjin Park, Ye Kyung Lee, Ok Kyu Song, Nicolas Locker, Sung-Gil Chi, Yoon Ki Kim

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

In the cytoplasm of mammalian cells, either cap-binding proteins 80 and 20 (CBP80/20) or eukaryotic translation initiation factor (eIF) 4E can direct the initiation of translation. Although the recruitment of ribosomes to mRNAs during eIF4E-dependent translation (ET) is well characterized, the molecular mechanism for CBP80/20-dependent translation (CT) remains obscure. Here, we show that CBP80/20-dependent translation initiation factor (CTIF), which has been shown to be preferentially involved in CT but not ET, specifically interacts with eIF3g, a component of the eIF3 complex involved in ribosome recruitment. By interacting with eIF3g, CTIF serves as an adaptor protein to bridge the CBP80/20 and the eIF3 complex, leading to efficient ribosome recruitment during CT. Accordingly, down-regulation of CTIF using a small interfering RNA causes a redistribution of CBP80 from polysome fractions to subpolysome fractions, without significant consequence to eIF4E distribution. In addition, down-regulation of eIF3g inhibits the efficiency of nonsense-mediated mRNA decay, which is tightly coupled to CT but not to ET. Moreover, the artificial tethering of CTIF to an intercistronic region of dicistronic mRNA results in translation of the downstream cistron in an eIF3-dependent manner. These findings support the idea that CT mechanistically differs from ET.

Original languageEnglish
Pages (from-to)18500-18509
Number of pages10
JournalJournal of Biological Chemistry
Volume287
Issue number22
DOIs
Publication statusPublished - 2012 May 25

Fingerprint

Nuclear Cap-Binding Protein Complex
RNA Cap-Binding Proteins
Eukaryotic Initiation Factors
Peptide Initiation Factors
Ribosomes
Messenger RNA
Down-Regulation
Nonsense Mediated mRNA Decay
Eukaryotic Initiation Factor-4E
Intergenic DNA
Polyribosomes
Small Interfering RNA
Cytoplasm
Cells
Genes
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Translation initiation on mRNAs bound by nuclear cap-binding protein complex CBP80/20 requires interaction between CBP80/20-dependent translation initiation factor and eukaryotic translation initiation factor 3g. / Choe, Junho; Oh, Nara; Park, Sungjin; Lee, Ye Kyung; Song, Ok Kyu; Locker, Nicolas; Chi, Sung-Gil; Kim, Yoon Ki.

In: Journal of Biological Chemistry, Vol. 287, No. 22, 25.05.2012, p. 18500-18509.

Research output: Contribution to journalArticle

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