Translation of polioviral mRNA is inhibited by cleavage of polypyrimidine tract-binding proteins executed by polioviral 3Cpro

Sung Hoon Back, Yoon Ki Kim, Woo Jae Kim, Sungchan Cho, Hoe Rang Oh, Jung Eun Kim, Sung Key Jang

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

The translation of polioviral mRNA occurs through an internal ribosomal entry site (IRES). Several RNA-binding proteins, such as polypyrimidine tract-binding protein (PTB) and poly(rC)-binding protein (PCBP), are required for the poliovirus IRES-dependent translation. Here we report that a poliovirus protein, 3Cpro (and/or 3CDpro), cleaves PTB isoforms (PTB1, PTB2, and PTB4). Three 3Cpro target sites (one major target site and two minor target sites) exist in PTBs. PTB fragments generated by poliovirus infection are redistributed to the cytoplasm from the nucleus, where most of the intact PTBs are localized. Moreover, these PTB fragments inhibit polioviral IRES-dependent translation in a cell-based assay system. We speculate that the proteolytic cleavage of PTBs may contribute to the molecular switching from translation to replication of polioviral RNA.

Original languageEnglish
Pages (from-to)2529-2542
Number of pages14
JournalJournal of Virology
Volume76
Issue number5
DOIs
Publication statusPublished - 2002 Feb 26
Externally publishedYes

ASJC Scopus subject areas

  • Immunology

Fingerprint Dive into the research topics of 'Translation of polioviral mRNA is inhibited by cleavage of polypyrimidine tract-binding proteins executed by polioviral 3C<sup>pro</sup>'. Together they form a unique fingerprint.

  • Cite this